CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018748
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 UBX domain-containing protein 4 
Protein Synonyms/Alias
 Erasin; UBX domain-containing protein 2 
Gene Name
 UBXN4 
Gene Synonyms/Alias
 KIAA0242; UBXD2; UBXDC1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
16PAAIATAKRSGAVFVubiquitination[1]
239GKEMLDYKRKQEEELubiquitination[1]
269RAARERIKQQIALDRubiquitination[1, 2]
285ERAARFAKTKEEVEAubiquitination[1, 2]
294KEEVEAAKAAALLAKubiquitination[1, 2]
301KAAALLAKQAEMEVKmethylation[3]
301KAAALLAKQAEMEVKubiquitination[1, 2, 4]
308KQAEMEVKRESYAREubiquitination[1]
373FPRREFTKEDYKKKLubiquitination[1, 2, 5]
459PNPASSSKSEKREPVubiquitination[1, 2, 4, 5]
462ASSSKSEKREPVRKRubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Involved in endoplasmic reticulum-associated protein degradation (ERAD). 
Sequence Annotation
 DOMAIN 315 393 UBX.
 MOD_RES 489 489 Phosphothreonine.  
Keyword
 3D-structure; Complete proteome; Endoplasmic reticulum; Membrane; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Unfolded protein response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 508 AA 
Protein Sequence
MLWFQGAIPA AIATAKRSGA VFVVFVAGDD EQSTQMAASW EDDKVTEASS NSFVAIKIDT 60
KSEACLQFSQ IYPVVCVPSS FFIGDSGIPL EVIAGSVSAD ELVTRIHKVR QMHLLKSETS 120
VANGSQSESS VSTPSASFEP NNTCENSQSR NAELCEIPPT SDTKSDTATG GESAGHATSS 180
QEPSGCSDQR PAEDLNIRVE RLTKKLEERR EEKRKEEEQR EIKKEIERRK TGKEMLDYKR 240
KQEEELTKRM LEERNREKAE DRAARERIKQ QIALDRAERA ARFAKTKEEV EAAKAAALLA 300
KQAEMEVKRE SYARERSTVA RIQFRLPDGS SFTNQFPSDA PLEEARQFAA QTVGNTYGNF 360
SLATMFPRRE FTKEDYKKKL LDLELAPSAS VVLLPAGRPT ASIVHSSSGD IWTLLGTVLY 420
PFLAIWRLIS NFLFSNPPPT QTSVRVTSSE PPNPASSSKS EKREPVRKRV LEKRGDDFKK 480
EGKIYRLRTQ DDGEDENNTW NGNSTQQM 508 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:HPA.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0005635; C:nuclear envelope; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0006986; P:response to unfolded protein; IEA:UniProtKB-KW. 
Interpro
 IPR012336; Thioredoxin-like_fold.
 IPR001012; UBX. 
Pfam
 PF00789; UBX 
SMART
 SM00166; UBX 
PROSITE
 PS50033; UBX 
PRINTS