CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002629
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Orotidine 5'-phosphate decarboxylase 
Protein Synonyms/Alias
 OMP decarboxylase; OMPDCase; OMPdecase 
Gene Name
 pyrF 
Gene Synonyms/Alias
 b1281; JW1273 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
35DALAFVDKIDPRDCRacetylation[1]
47DCRLKVGKEMFTLFGacetylation[1]
185QVFGQEFKLVTPGIRacetylation[1]
235VDPAQTLKAINASLQacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP). 
Sequence Annotation
 REGION 71 80 Substrate binding.
 ACT_SITE 73 73 Proton donor.
 BINDING 22 22 Substrate.
 BINDING 44 44 Substrate.
 BINDING 131 131 Substrate.
 BINDING 192 192 Substrate.
 BINDING 201 201 Substrate.
 BINDING 221 221 Substrate; via amide nitrogen.
 BINDING 222 222 Substrate.  
Keyword
 3D-structure; Complete proteome; Decarboxylase; Lyase; Pyrimidine biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 245 AA 
Protein Sequence
MTLTASSSSR AVTNSPVVVA LDYHNRDDAL AFVDKIDPRD CRLKVGKEMF TLFGPQFVRE 60
LQQRGFDIFL DLKFHDIPNT AAHAVAAAAD LGVWMVNVHA SGGARMMTAA REALVPFGKD 120
APLLIAVTVL TSMEASDLVD LGMTLSPADY AERLAALTQK CGLDGVVCSA QEAVRFKQVF 180
GQEFKLVTPG IRPQGSEAGD QRRIMTPEQA LSAGVDYMVI GRPVTQSVDP AQTLKAINAS 240
LQRSA 245 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:EcoliWiki.
 GO:0004590; F:orotidine-5'-phosphate decarboxylase activity; IDA:EcoCyc.
 GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IMP:EcoCyc.
 GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0015949; P:nucleobase-containing small molecule interconversion; IDA:EcoliWiki. 
Interpro
 IPR013785; Aldolase_TIM.
 IPR014732; OMPdecase.
 IPR018089; OMPdecase_AS.
 IPR001754; OMPdeCOase_dom.
 IPR011060; RibuloseP-bd_barrel. 
Pfam
 PF00215; OMPdecase 
SMART
 SM00934; OMPdecase 
PROSITE
 PS00156; OMPDECASE 
PRINTS