CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012121
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphatidylinositol-binding clathrin assembly protein 
Protein Synonyms/Alias
 Clathrin assembly lymphoid myeloid leukemia protein 
Gene Name
 PICALM 
Gene Synonyms/Alias
 CALM 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
134YSRYLNEKAVSYRQVubiquitination[1]
252TRISEFLKVAEQVGIubiquitination[1]
318STGLSLTKVDEREKQubiquitination[1, 2, 3, 4]
324TKVDEREKQAALEEEubiquitination[1]
570NWSQPGEKKLTGGSNubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Assembly protein recruiting clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. 
Sequence Annotation
 DOMAIN 14 145 ENTH.
 REGION 221 294 Interaction with FAM64A.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 443 443 Phosphoserine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Chromosomal rearrangement; Coated pit; Complete proteome; Cytoplasmic vesicle; Endocytosis; Golgi apparatus; Membrane; Nucleus; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 652 AA 
Protein Sequence
MSGQSLTDRI TAAQHSVTGS AVSKTVCKAT THEIMGPKKK HLDYLIQCTN EMNVNIPQLA 60
DSLFERTTNS SWVVVFKSLI TTHHLMVYGN ERFIQYLASR NTLFNLSNFL DKSGLQGYDM 120
STFIRRYSRY LNEKAVSYRQ VAFDFTKVKR GADGVMRTMN TEKLLKTVPI IQNQMDALLD 180
FNVNSNELTN GVINAAFMLL FKDAIRLFAA YNEGIINLLE KYFDMKKNQC KEGLDIYKKF 240
LTRMTRISEF LKVAEQVGID RGDIPDLSQA PSSLLDALEQ HLASLEGKKI KDSTAASRAT 300
TLSNAVSSLA STGLSLTKVD EREKQAALEE EQARLKALKE QRLKELAKKP HTSLTTAASP 360
VSTSAGGIMT APAIDIFSTP SSSNSTSKLP NDLLDLQQPT FHPSVHPMST ASQVASTWGD 420
PFSATVDAVD DAIPSLNPFL TKSSGDVHLS ISSDVSTFTT RTPTHEMFVG FTPSPVAQPH 480
PSAGLNVDFE SVFGNKSTNV IVDSGGFDEL GGLLKPTVAS QNQNLPVAKL PPSKLVSDDL 540
DSSLANLVGN LGIGNGTTKN DVNWSQPGEK KLTGGSNWQP KVAPTTAWNA ATMAPPVMAY 600
PATTPTGMIG YGIPPQMGSV PVMTQPTLIY SQPVMRPPNP FGPVSGAQIQ FM 652 
Gene Ontology
 GO:0030118; C:clathrin coat; IEA:InterPro.
 GO:0030136; C:clathrin-coated vesicle; IEA:UniProtKB-SubCell.
 GO:0005905; C:coated pit; IDA:UniProtKB.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:BHF-UCL.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0045211; C:postsynaptic membrane; ISS:BHF-UCL.
 GO:0042734; C:presynaptic membrane; ISS:BHF-UCL.
 GO:0005545; F:1-phosphatidylinositol binding; ISS:UniProtKB.
 GO:0032050; F:clathrin heavy chain binding; IDA:BHF-UCL.
 GO:0007409; P:axonogenesis; IEA:Compara.
 GO:0048268; P:clathrin coat assembly; IMP:BHF-UCL.
 GO:0048813; P:dendrite morphogenesis; IEA:Compara.
 GO:0016197; P:endosomal transport; IMP:BHF-UCL.
 GO:0030097; P:hemopoiesis; IEA:Compara.
 GO:0048261; P:negative regulation of receptor-mediated endocytosis; IDA:BHF-UCL.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:BHF-UCL.
 GO:0031623; P:receptor internalization; IMP:BHF-UCL.
 GO:0032880; P:regulation of protein localization; IDA:BHF-UCL. 
Interpro
 IPR011417; ANTH_dom.
 IPR014712; Clathrin_Pinositid-bd_GAT-like.
 IPR008942; ENTH_VHS.
 IPR013809; Epsin-like_N. 
Pfam
 PF07651; ANTH 
SMART
 SM00273; ENTH 
PROSITE
 PS50942; ENTH 
PRINTS