CPLM-007753

Genbank Nucleotide ID

Voltage-dependent anion-selective channel protein 2

Protein Synonyms/Alias

VDAC-2; hVDAC2; Outer mitochondrial membrane protein porin 2

Homo sapiens (Human)

Position	Peptide	Type	References
31	AARDIFNKGFGFGLV	acetylation	[1]
31	AARDIFNKGFGFGLV	ubiquitination	[2, 3, 4, 5, 6]
39	GFGFGLVKLDVKTKS	acetylation	[1, 7]
39	GFGFGLVKLDVKTKS	ubiquitination	[2]
72	VTGTLETKYKWCEYG	acetylation	[1]
72	VTGTLETKYKWCEYG	ubiquitination	[3, 6]
74	GTLETKYKWCEYGLT	acetylation	[1]
120	TFSPNTGKKSGKIKS	ubiquitination	[2, 3, 5]
235	TRFGIAAKYQLDPTA	ubiquitination	[3]
285	SINAGGHKVGLALEL	ubiquitination	[2, 3]

[1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[7] Regulation of cellular metabolism by protein lysine acetylation.
Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]

Functional Description

Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation- selective.

NP_BIND 253 255 NAD (By similarity).
NP_BIND 271 275 NAD (By similarity).
MOD_RES 2 2 N-acetylalanine.
MOD_RES 31 31 N6-acetyllysine.

Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Ion transport; Membrane; Mitochondrion; Mitochondrion outer membrane; NAD; Nucleotide-binding; Polymorphism; Porin; Reference proteome; Transmembrane; Transmembrane beta strand; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
GO:0042645; C:mitochondrial nucleoid; IDA:BHF-UCL.
GO:0005741; C:mitochondrial outer membrane; TAS:ProtInc.
GO:0046930; C:pore complex; IEA:UniProtKB-KW.
GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
GO:0015288; F:porin activity; IEA:UniProtKB-KW.
GO:0008308; F:voltage-gated anion channel activity; TAS:UniProtKB.
GO:2001243; P:negative regulation of intrinsic apoptotic signaling pathway; IEA:Compara.
GO:0032272; P:negative regulation of protein polymerization; IEA:Compara.

IPR023614; Porin_dom.
IPR001925; Porin_Euk.
IPR027246; Porin_Euk/Tom40.

PS00558; EUKARYOTIC_PORIN

PR00185; EUKARYTPORIN.