CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006705
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NADPH--cytochrome P450 reductase 
Protein Synonyms/Alias
 CPR; P450R 
Gene Name
 Por 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
56EEIPEFSKIQTTAPPubiquitination[1]
65QTTAPPVKESSFVEKubiquitination[1]
176AVFGLGNKTYEHFNAubiquitination[1]
186EHFNAMGKYVDQRLEacetylation[2]
186EHFNAMGKYVDQRLEubiquitination[1]
257HEDMDTAKVYTGEMGubiquitination[1]
273LKSYENQKPPFDAKNubiquitination[1]
279QKPPFDAKNPFLAAVacetylation[2]
279QKPPFDAKNPFLAAVubiquitination[1]
311ELDISDSKIRYESGDubiquitination[1]
358LDEESNKKHPFPCPTubiquitination[1]
404SEQEHLHKMASSSGEubiquitination[1]
487AKSGRVNKGVATSWLubiquitination[1]
523SQFRLPFKPTTPVIMubiquitination[1]
555AWLREQGKEVGETLLacetylation[3]
555AWLREQGKEVGETLLubiquitination[1]
584EELARFHKDGALTQLubiquitination[1]
610VYVQHLLKRDKEHLWubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 
Functional Description
 This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. 
Sequence Annotation
 DOMAIN 80 224 Flavodoxin-like.
 DOMAIN 279 521 FAD-binding FR-type.
 NP_BIND 170 201 FMN (By similarity).
 NP_BIND 314 325 FAD (By similarity).
 NP_BIND 451 461 FAD (By similarity).
 NP_BIND 530 548 NADP (By similarity).
 NP_BIND 625 641 NADP (By similarity).
 MOD_RES 2 2 N-acetylglycine (By similarity).
 MOD_RES 575 575 Phosphotyrosine.  
Keyword
 Acetylation; Complete proteome; Endoplasmic reticulum; FAD; Flavoprotein; FMN; Membrane; NADP; Oxidoreductase; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 678 AA 
Protein Sequence
MGDSHEDTSA TVPEAVAEEV SLFSTTDIVL FSLIVGVLTY WFIFKKKKEE IPEFSKIQTT 60
APPVKESSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK DAHRYGMRGM SADPEEYDLA 120
DLSSLPEIDK SLVVFCMATY GEGDPTDNAQ DFYDWLQETD VDLTGVKFAV FGLGNKTYEH 180
FNAMGKYVDQ RLEQLGAQRI FELGLGDDDG NLEEDFITWR EQFWPAVCEF FGVEATGEES 240
SIRQYELVVH EDMDTAKVYT GEMGRLKSYE NQKPPFDAKN PFLAAVTTNR KLNQGTERHL 300
MHLELDISDS KIRYESGDHV AVYPANDSTL VNQIGEILGA DLDVIMSLNN LDEESNKKHP 360
FPCPTTYRTA LTYYLDITNP PRTNVLYELA QYASEPSEQE HLHKMASSSG EGKELYLSWV 420
VEARRHILAI LQDYPSLRPP IDHLCELLPR LQARYYSIAS SSKVHPNSVH ICAVAVEYEA 480
KSGRVNKGVA TSWLRTKEPA GENGRRALVP MFVRKSQFRL PFKPTTPVIM VGPGTGVAPF 540
MGFIQERAWL REQGKEVGET LLYYGCRRSD EDYLYREELA RFHKDGALTQ LNVAFSREQA 600
HKVYVQHLLK RDKEHLWKLI HEGGAHIYVC GDARNMAKDV QNTFYDIVAE FGPMEHTQAV 660
DYVKKLMTKG RYSLDVWS 678 
Gene Ontology
 GO:0005829; C:cytosol; IBA:RefGenome.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0004128; F:cytochrome-b5 reductase activity, acting on NAD(P)H; IEA:Compara.
 GO:0009055; F:electron carrier activity; IEA:Compara.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:Compara.
 GO:0010181; F:FMN binding; IEA:Compara.
 GO:0016787; F:hydrolase activity; IEA:Compara.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0047726; F:iron-cytochrome-c reductase activity; IEA:Compara.
 GO:0050661; F:NADP binding; IEA:Compara.
 GO:0003958; F:NADPH-hemoprotein reductase activity; IBA:RefGenome.
 GO:0016709; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen; IBA:RefGenome.
 GO:0009437; P:carnitine metabolic process; IEA:Compara.
 GO:0090346; P:cellular organofluorine metabolic process; IEA:Compara.
 GO:0071372; P:cellular response to follicle-stimulating hormone stimulus; IEA:Compara.
 GO:0071375; P:cellular response to peptide hormone stimulus; IEA:Compara.
 GO:0070988; P:demethylation; IEA:Compara.
 GO:0019395; P:fatty acid oxidation; IEA:Compara.
 GO:0009812; P:flavonoid metabolic process; IEA:Compara.
 GO:0018393; P:internal peptidyl-lysine acetylation; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; IEA:Compara.
 GO:0060192; P:negative regulation of lipase activity; IEA:Compara.
 GO:0043602; P:nitrate catabolic process; IEA:Compara.
 GO:0046210; P:nitric oxide catabolic process; IEA:Compara.
 GO:0045542; P:positive regulation of cholesterol biosynthetic process; IEA:Compara.
 GO:0032332; P:positive regulation of chondrocyte differentiation; IEA:Compara.
 GO:0032770; P:positive regulation of monooxygenase activity; IEA:Compara.
 GO:0045880; P:positive regulation of smoothened signaling pathway; IEA:Compara.
 GO:0090031; P:positive regulation of steroid hormone biosynthetic process; IEA:Compara.
 GO:0003420; P:regulation of growth plate cartilage chondrocyte proliferation; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0007584; P:response to nutrient; IEA:Compara. 
Interpro
 IPR003097; FAD-binding_1.
 IPR017927; Fd_Rdtase_FAD-bd.
 IPR001094; Flavdoxin.
 IPR008254; Flavodoxin/NO_synth.
 IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
 IPR023173; NADPH_Cyt_P450_Rdtase_dom3.
 IPR001433; OxRdtase_FAD/NAD-bd.
 IPR023208; P450R.
 IPR017938; Riboflavin_synthase-like_b-brl. 
Pfam
 PF00667; FAD_binding_1
 PF00258; Flavodoxin_1
 PF00175; NAD_binding_1 
SMART
  
PROSITE
 PS51384; FAD_FR
 PS50902; FLAVODOXIN_LIKE 
PRINTS
 PR00369; FLAVODOXIN.
 PR00371; FPNCR.