CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022125
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bromodomain and WD repeat-containing protein 1 
Protein Synonyms/Alias
 WD repeat-containing protein 9 
Gene Name
 BRWD1 
Gene Synonyms/Alias
 C21orf107; WDR9 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
50EQYQLLPKRLDWEGNubiquitination[1]
1037ATGKLMDKSFSIRYHubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 May be a transcriptional activator. May be involved in chromatin remodeling (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. 
Sequence Annotation
 REPEAT 184 223 WD 1.
 REPEAT 226 265 WD 2.
 REPEAT 268 311 WD 3.
 REPEAT 322 365 WD 4.
 REPEAT 366 405 WD 5.
 REPEAT 424 463 WD 6.
 REPEAT 466 506 WD 7.
 REPEAT 514 553 WD 8.
 DOMAIN 1177 1247 Bromo 1.
 DOMAIN 1330 1400 Bromo 2.
 MOD_RES 696 696 Phosphoserine.
 MOD_RES 701 701 Phosphoserine.
 MOD_RES 710 710 Phosphoserine.
 MOD_RES 1289 1289 Phosphoserine.
 MOD_RES 1475 1475 Phosphoserine.
 MOD_RES 1477 1477 Phosphothreonine.
 MOD_RES 1479 1479 Phosphoserine.
 MOD_RES 1605 1605 Phosphoserine.
 MOD_RES 1607 1607 Phosphoserine.
 MOD_RES 1678 1678 Phosphoserine.
 MOD_RES 1683 1683 Phosphoserine.
 MOD_RES 1686 1686 Phosphoserine.
 MOD_RES 1755 1755 Phosphoserine (By similarity).
 MOD_RES 1756 1756 Phosphoserine (By similarity).
 MOD_RES 1786 1786 Phosphoserine (By similarity).
 MOD_RES 1788 1788 Phosphoserine (By similarity).
 MOD_RES 1793 1793 Phosphoserine (By similarity).
 MOD_RES 1904 1904 Phosphoserine.
 MOD_RES 1905 1905 Phosphoserine.
 MOD_RES 1907 1907 Phosphoserine.
 MOD_RES 1910 1910 Phosphoserine.
 MOD_RES 2018 2018 Phosphoserine.
 MOD_RES 2020 2020 Phosphoserine.
 MOD_RES 2052 2052 Phosphoserine (By similarity).  
Keyword
 3D-structure; Activator; Alternative splicing; Bromodomain; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2320 AA 
Protein Sequence
MAEPSSARRP VPLIESELYF LIARYLSAGP CRRAAQVLVQ ELEQYQLLPK RLDWEGNEHN 60
RSYEELVLSN KHVAPDHLLQ ICQRIGPMLD KEIPPSISRV TSLLGAGRQS LLRTAKDCRH 120
TVWKGSAFAA LHRGRPPEMP VNYGSPPNLV EIHRGKQLTG CSTFSTAFPG TMYQHIKMHR 180
RILGHLSAVY CVAFDRTGHR IFTGSDDCLV KIWSTHNGRL LSTLRGHSAE ISDMAVNYEN 240
TMIAAGSCDK IIRVWCLRTC APVAVLQGHT GSITSLQFSP MAKGSQRYMV STGADGTVCF 300
WQWDLESLKF SPRPLKFTEK PRPGVQMLCS SFSVGGMFLA TGSTDHVIRM YFLGFEAPEK 360
IAELESHTDK VDSIQFCNNG DRFLSGSRDG TARIWRFEQL EWRSILLDMA TRISGDLSSE 420
EERFMKPKVT MIAWNQNDSI VVTAVNDHVL KVWNSYTGQL LHNLMGHADE VFVLETHPFD 480
SRIMLSAGHD GSIFIWDITK GTKMKHYFNM IEGQGHGAVF DCKFSQDGQH FACTDSHGHL 540
LIFGFGCSKP YEKIPDQMFF HTDYRPLIRD SNNYVLDEQT QQAPHLMPPP FLVDVDGNPH 600
PTKYQRLVPG RENSADEHLI PQLGYVATSD GEVIEQIISL QTNDNDERSP ESSILDGMIR 660
QLQQQQDQRM GADQDTIPRG LSNGEETPRR GFRRLSLDIQ SPPNIGLRRS GQVEGVRQMH 720
QNAPRSQIAT ERDLQAWKRR VVVPEVPLGI FRKLEDFRLE KGEEERNLYI IGRKRKTLQL 780
SHKSDSVVLV SQSRQRTCRR KYPNYGRRNR SWRELSSGNE SSSSVRHETS CDQSEGSGSS 840
EEDEWRSDRK SESYSESSSD SSSRYSDWTA DAGINLQPPL RTSCRRRITR FCSSSEDEIS 900
TENLSPPKRR RKRKKENKPK KENLRRMTPA ELANMEHLYE FHPPVWITDT TLRKSPFVPQ 960
MGDEVIYFRQ GHEAYIEAVR RNNIYELNPN KEPWRKMDLR DQELVKIVGI RYEVGPPTLC 1020
CLKLAFIDPA TGKLMDKSFS IRYHDMPDVI DFLVLRQFYD EARQRNWQSC DRFRSIIDDA 1080
WWFGTVLSQE PYQPQYPDSH FQCYIVRWDN TEIEKLSPWD MEPIPDNVDP PEELGASISV 1140
TTDELEKLLY KPQAGEWGQK SRDEECDRII SGIDQLLNLD IAAAFAGPVD LCTYPKYCTV 1200
VAYPTDLYTI RMRLVNRFYR RLSALVWEVR YIEHNARTFN EPESVIARSA KKITDQLLKF 1260
IKNQHCTNIS ELSNTSENDE QNAEDLDDSD LPKTSSGRRR VHDGKKSIRA TNYVESNWKK 1320
QCKELVNLIF QCEDSEPFRQ PVDLVEYPDY RDIIDTPMDF GTVRETLDAG NYDSPLEFCK 1380
DIRLIFSNAK AYTPNKRSKI YSMTLRLSAL FEEKMKKISS DFKIGQKFNE KLRRSQRFKQ 1440
RQNCKGDSQP NKSIRNLKPK RLKSQTKIIP ELVGSPTQST SSRTAYLGTH KTSAGISSGV 1500
TSGDSSDSAE SSERRKRNRP ITNGSTLSES EVEDSLATSL SSSASSSSEE SKESSRARES 1560
SSRSGLSRSS NLRVTRTRAA QRKTGPVSLA NGCGRKATRK RVYLSDSDNN SLETGEILKA 1620
RAGNNRKVLR KCAAVAANKI KLMSDVEENS SSESVCSGRK LPHRNASAVA RKKLLHNSED 1680
EQSLKSEIEE EELKDENQLL PVSSSHTAQS NVDESENRDS ESESDLRVAR KNWHANGYKS 1740
HTPAPSKTKF LKIESSEEDS KSHDSDHACN RTAGPSTSVQ KLKAESISEE ADSEPGRSGG 1800
RKYNTFHKNA SFFKKTKILS DSEDSESEEQ DREDGKCHKM EMNPISGNLN CDPIAMSQCS 1860
SDHGCETDLD SDDDKIEKPN NFMKDSASQD NGLSRKISRK RVCSSDSDSS LQVVKKSSKA 1920
RTGLLRITRR CAATAANKIK LMSDVEDVSL ENVHTRSKNG RKKPLHLACT TAKKKLSDCE 1980
GSVHCEVPSE QYACEGKPPD PDSEGSTKVL SQALNGDSDS EDMLNSEHKH RHTNIHKIDA 2040
PSKRKSSSVT SSGEDSKSHI PGSETDRTFS SESTLAQKAT AENNFEVELN YGLRRWNGRR 2100
LRTYGKAPFS KTKVIHDSQE TAEKEVKRKR SHPELENVKI SETTGNSKFR PDTSSKSSDL 2160
GSVTESDIDC TDNTKTKRRK TKGKAKVVRK EFVPRDREPN TKVRTCMHNQ KDAVQMPSET 2220
LKAKMVPEKV PRRCATVAAN KIKIMSNLKE TISGPENVWI RKSSRKLPHR NASAAAKKKL 2280
LNVYKEDDTT INSESEKELE DINRKMLFLR GFRSWKENAQ 2320 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0007010; P:cytoskeleton organization; IMP:UniProtKB.
 GO:0008360; P:regulation of cell shape; IMP:UniProtKB.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001487; Bromodomain.
 IPR018359; Bromodomain_CS.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00439; Bromodomain
 PF00400; WD40 
SMART
 SM00297; BROMO
 SM00320; WD40 
PROSITE
 PS00633; BROMODOMAIN_1
 PS50014; BROMODOMAIN_2
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00503; BROMODOMAIN.