CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-038983
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Malic enzyme 
Protein Synonyms/Alias
  
Gene Name
 Me1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
37IQVLRVIKNFERLNSacetylation[1]
235NAFRLLNKYRNKYCTacetylation[1]
314KIWLVDSKGLIVKGRacetylation[1]
328RASLTEEKEVFAHEHacetylation[1]
339AHEHEEMKNLEAIVQacetylation[1]
493VSLKIAVKIVQDAYKacetylation[1]
493VSLKIAVKIVQDAYKubiquitination[2]
500KIVQDAYKEKMATVYacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
  
Sequence Annotation
 ACT_SITE 79 79 Proton donor (By similarity).
 ACT_SITE 102 102 Proton donor (By similarity).
 ACT_SITE 150 150 Proton acceptor (By similarity).
 ACT_SITE 173 173 Proton acceptor (By similarity).
 METAL 222 222 Divalent metal cation (By similarity).
 METAL 223 223 Divalent metal cation (By similarity).
 METAL 246 246 Divalent metal cation (By similarity).  
Keyword
 Complete proteome; Metal-binding; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 549 AA 
Protein Sequence
LSQDLAFTLE ERQQLNIHGL LPPCIVNQEI QVLRVIKNFE RLNSDFDRYL LLMDLQDRNE 60
KLFYSVLMSN VEKFMPIVYT PTVGLACQQY SLAFRKPRGL FISIHDKGHI ASVLNAWPED 120
VVKAIVVTDG ERILGLGDLG CNGMGIPVGK LALYTACGGV NPQQCLPITL DVGTENEELL 180
KDPLYIGLRH RRVRGPEYDA FLDEFMEAAS SKYGMNCLIQ FEDFANLNAF RLLNKYRNKY 240
CTFNDDIQGT ASVAVAGLLA ALRITKNKLS DQTVLFQGAG EAALGIAHLI VMAMEKEGLS 300
KEKARQKIWL VDSKGLIVKG RASLTEEKEV FAHEHEEMKN LEAIVQKIKP TALIGVAAIG 360
GAFTEQILKD MAAFNERPII FALSNPTSKA ECSAEECYKV TKGRAIFASG SPFDPVTLPD 420
GRTLFPGQGN NSYVFPGVAL GVVACGLRHI NDSVFLTTAE VISQQVSDKH LEEGRLYPPL 480
NTIRDVSLKI AVKIVQDAYK EKMATVYPEP QNKEEFVSSQ MYSTNYDQIL PDCYSWPEEV 540
QKIQTKVNQ 549 
Gene Ontology
 GO:0016619; F:malate dehydrogenase (oxaloacetate-decarboxylating) activity; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0006108; P:malate metabolic process; IEA:InterPro. 
Interpro
 IPR015884; Malic_enzyme_CS.
 IPR012301; Malic_N.
 IPR012302; Malic_NAD-bd.
 IPR001891; Malic_OxRdtase.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00390; malic
 PF03949; Malic_M 
SMART
 SM00919; Malic_M 
PROSITE
 PS00331; MALIC_ENZYMES 
PRINTS
 PR00072; MALOXRDTASE.