CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-028561
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Histone-lysine N-methyltransferase EHMT2 
Protein Synonyms/Alias
  
Gene Name
 EHMT2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
286SDLAKRRKLNSGGGLubiquitination[1]
657PGREALEKALVIQESubiquitination[1, 2]
682RQLYLSVKQGELQKVubiquitination[2]
708FQSDQQSKRTPLHAAubiquitination[2]
770RGGCVYSKEEDGSTCubiquitination[2, 3]
905ANPELRNKEGDTAWDubiquitination[1, 2, 4]
943NRAIRTEKIICRDVAubiquitination[2]
974EPCPEDYKYISENCEubiquitination[3]
1021SIRCWYDKDGRLLQEubiquitination[2]
1070LQLYRTAKMGWGVRAubiquitination[1, 2, 5]
1187RFWDIKSKYFTCQCGubiquitination[1, 2]
1197TCQCGSEKCKHSAEAubiquitination[1, 2]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 Chromosome; Complete proteome; Methyltransferase; Nucleus; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1233 AA 
Protein Sequence
MRGLPRGRGL MRARGRGRAA PPGSRGRGRG GPHRGRGRPR SLLSLPRAQA SWTPQLSTGL 60
TSPPVPCLPS QGEAPAEMGA LLLEKETRGA TERVHGSLGD TPRSEETLPK ATPDSLEPAG 120
PSSPASVTVT VGDEGADTPV GATPLIGDES ENLEGDGDLR GGRILLGHAT KSFPSSPSKG 180
GSCPSRAKMS MTGAGKSPPS VQSLAMRLLS MPGAQGAAAA GSEPPPATTS PEGQPKVHRA 240
RKTMSKPGNG QPPVPEKRPP EIQHFRMSDD VHSLGKVTSD LAKRRKLNSG GGLSEELGSA 300
RRSGEVTLTK GDPGSLEEWE TVVGDDFSLY YDSYSVDERV DSDSKSEVEA LTEQLSEEEE 360
EEEEEEEEEE EEEEEEEEEE DEESGNQSDR SGSSGRRKAK KKWRKDSPWV KPSRKRRKRE 420
PPRAKEPRGV SNDTSSLETE RGFEELPLCS CRMEAPKIDR ISERAGHKCM ATESVDGELS 480
GCNAAILKRE TMRPSSRVAL MVLCETHRAR MVKHHCCPGC GYFCTAGTFL ECHPDFRVAH 540
RFHKACVSQL NGMVFCPHCG EDASEAQEVT IPRGDGVTPP AGTAAPAPPP LSQDVPGRAD 600
TSQPSARMRG HGEPRRPPCD PLADTIDSSG PSLTLPNGGC LSAVGLPLGP GREALEKALV 660
IQESERRKKL RFHPRQLYLS VKQGELQKVI LMLLDNLDPN FQSDQQSKRT PLHAAAQKGS 720
VEICHVLLQA GANINAVDKQ QRTPLMEAVV NNHLEVARYM VQRGGCVYSK EEDGSTCLHH 780
AAKIGNLEMV SLLLSTGQVD VNAQDSGGWT PIIWAAEHKH IEVIRMLLTR GADVTLTDNE 840
ENICLHWASF TGSAAIAEVL LNARCDLHAV NYHGDTPLHI AARESYHDCV LLFLSRGANP 900
ELRNKEGDTA WDLTPERSDV WFALQLNRKL RLGVGNRAIR TEKIICRDVA RGYENVPIPC 960
VNGVDGEPCP EDYKYISENC ETSTMNIDRN ITHLQHCTCV DDCSSSNCLC GQLSIRCWYD 1020
KDGRLLQEFN KIEPPLIFEC NQACSCWRNC KNRVVQSGIK VRLQLYRTAK MGWGVRALQT 1080
IPQGTFICEY VGELISDAEA DVREDDSYLF DLDNKDGEVY CIDARYYGNI SRFINHLCDP 1140
NIIPVRVFML HQDLRFPRIA FFSSRDIRTG EELGFDYGDR FWDIKSKYFT CQCGSEKCKH 1200
SAEAIALEQS RLARLDPHPE LLPELGSLPP VNT 1233 
Gene Ontology
 GO:0005694; C:chromosome; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IEA:UniProtKB-KW.
 GO:0018024; F:histone-lysine N-methyltransferase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR003616; Post-SET_dom.
 IPR007728; Pre-SET_dom.
 IPR003606; Pre-SET_Zn-bd_sub.
 IPR001214; SET_dom. 
Pfam
 PF12796; Ank_2
 PF05033; Pre-SET
 PF00856; SET 
SMART
 SM00248; ANK
 SM00508; PostSET
 SM00468; PreSET
 SM00317; SET 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50867; PRE_SET
 PS50280; SET 
PRINTS
 PR01415; ANKYRIN.