CPLM-013437

Genbank Nucleotide ID

Programmed cell death protein 4

Protein Synonyms/Alias

Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a

Homo sapiens (Human)

Position	Peptide	Type	References
166	LDERAFEKTLTPIIQ	ubiquitination	[1, 2]
197	DLNLGEMKSGVPVLA	ubiquitination	[2]
242	DVEKSFDKLLKDLPE	ubiquitination	[3, 4]
245	KSFDKLLKDLPELAL	ubiquitination	[1, 2]
283	NTYIDSYKGTVDCVQ	ubiquitination	[1, 5]
297	QARAALDKATVLLSM	ubiquitination	[2]
306	TVLLSMSKGGKRKDS	ubiquitination	[2]
402	TITVDQMKRGYERIY	ubiquitination	[2]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]

Functional Description

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity).

DOMAIN 163 284 MI 1.
DOMAIN 326 449 MI 2.
MOTIF 58 64 Nuclear localization signal (Potential).
MOTIF 70 76 Phosphodegron.
MOTIF 241 250 Nuclear localization signal (Potential).
MOD_RES 1 1 N-acetylmethionine.
MOD_RES 67 67 Phosphoserine; by PKB and RPS6KB1.
MOD_RES 76 76 Phosphoserine.
MOD_RES 78 78 Phosphoserine.
MOD_RES 80 80 Phosphoserine.
MOD_RES 94 94 Phosphoserine.
MOD_RES 152 152 Phosphotyrosine.
MOD_RES 457 457 Phosphoserine; by PKB.

3D-structure; Acetylation; Alternative splicing; Apoptosis; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; RNA-binding; Tumor suppressor; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; IDA:UniProtKB.
GO:0005634; C:nucleus; IMP:UniProtKB.
GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
GO:0006915; P:apoptotic process; NAS:UniProtKB.
GO:0007569; P:cell aging; IDA:UniProtKB.
GO:0045786; P:negative regulation of cell cycle; NAS:UniProtKB.
GO:0043508; P:negative regulation of JUN kinase activity; ISS:UniProtKB.
GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
GO:0016070; P:RNA metabolic process; IEA:InterPro.

IPR016024; ARM-type_fold.
IPR003891; Initiation_fac_eIF4g_MI.
IPR016021; MIF4-like_typ_1/2/3.