CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006574
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dynein heavy chain, cytoplasmic 
Protein Synonyms/Alias
 Dynein heavy chain, cytosolic; DYHC 
Gene Name
 DYN1 
Gene Synonyms/Alias
 DHC1; YKR054C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
1026AQRHIRSKMDSWILYubiquitination[1]
1036SWILYVSKHLLTIYEubiquitination[1]
Reference
 [1] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269
Functional Description
 Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required to maintain uniform nuclear distribution in hyphae. May play an important role in the proper orientation of the mitotic spindle into the budding daughter cell yeast. Probably required for normal progression of the cell cycle. 
Sequence Annotation
 NP_BIND 1796 1803 ATP (Potential).
 NP_BIND 2074 2081 ATP (Potential).
 NP_BIND 2418 2425 ATP (Potential).
 NP_BIND 2760 2767 ATP (Potential).
 REGION 1 1757 Stem (By similarity).
 REGION 1758 1979 AAA 1 (By similarity).
 REGION 2036 2273 AAA 2 (By similarity).
 REGION 2379 2628 AAA 3 (By similarity).
 REGION 2722 2984 AAA 4 (By similarity).
 REGION 2993 3300 Stalk (By similarity).
 REGION 3370 3599 AAA 5 (By similarity).
 REGION 3760 3970 AAA 6 (By similarity).  
Keyword
 3D-structure; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Dynein; Karyogamy; Microtubule; Motor protein; Nucleotide-binding; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 4092 AA 
Protein Sequence
MCKNEARLAN ELIEFVAATV TGIKNSPKEN EQAFIDYLHC QYLERFQFFL GLLDGREFDT 60
LFVFLFEELD RTIVTIDIGE EAIYDANLAN KKYSTLLIIK SRSVIVDAEP IATQISAIYL 120
PGPVNAGNLA SIITHGVSSV FGQLIKSDTK TYSVETIDKT RRKLDDISKQ FQQLHTSIET 180
PDLLAMVPSI IKLAVSKGAT SHDYANYLPS NDLESMRFLN ILQSIANKWF LVLKQTLAID 240
RDIKNGSFLD EVEFWSNFYE VLKSLIEQTQ SQEFQVCLSV LTNAKRFHNL TNLLNEGSLS 300
DKFKLADKYN QFLSSIPIDE VRQASNLEDL QELFPVLASS LKKFRYSGYP VQRFVVLMDK 360
ISQEVMDAIL SNLSDLFQLE YGSFLGLYEK SAGMIEEWDD IVQDVNLLIR EDLRKRAPQE 420
LLIQKLTFTS ASVKATLDEI LSTRKRFFSL AETIKSISPS TYHEEIQRLY HPFEQIHDIS 480
VNFRLKLEQA ESEFSKNMLD LEKKLQNTLA SFMDSDHCPT EKLSYLVKFK PLMELCPRIK 540
VKVLENQQIL LLEIKKDIRQ LETGLELLPK ILHVEALNNI PPISARISYF LNVQSRIDNI 600
VQYLEALFGS NWNDTLEGRS ISTSIVQLRK ETNPHDVFLH WLGNFPEKAT ANLLTTPILK 660
LIRNNEDDYE LKVNFDFALA AAYSELRSLT YMAFQVPSHI VRIARTYMYL YPRAINLVEL 720
IQTFFSLSKS LSYTFYTNIF LKRNVQTVWL LLQQILITPW ESLQEESSEM SCSVHSLARL 780
EKSIDGILSD YQILKNSEPQ FAKEFSGLKS FDGTADDLHE VEEIISNIQA IFENLFTKGL 840
TNVSDHISTF NNLIISIILE KVRLNLKKMH FPKHVLKLSF NEGRITSSPS LAAMKRSLLK 900
DIEALLNKVV LINFLHDPDH PLSTTLTFNS LVIKLKDDIQ NCIEQVQNLH CKINSYVKEW 960
QKMEFLWQIT EEAFLEVVDN STQRCFGILK GLLDSQSKFD LIISRNNFSK NLVLHTEDAQ 1020
RHIRSKMDSW ILYVSKHLLT IYERDARKLH EDMNRDREAV EDMDINFTSL KNITVIIEAV 1080
NVNKRHLTER DIQIKLLGSV MRALTKLKVR FPSHFVYIDQ LDNDFSSLRQ SLSYVEQELQ 1140
KHRVVIAKSL EEGVENINNL SQSLNESWSV RKPISPTLTP PEALKILEFF NESITKLKKK 1200
MHSVAAAAKM LLIPVVLNDQ LTHVVEEVKT YDLVWRSIKN LWEDVQRTFE TPWCRVDVLL 1260
LQSDLANFLR RADELPRAVK QFEMYKSLFS QVNMLTSVNK ILVELKDGAL KPRHWNMIFR 1320
DIGKRQIQKN LLDKLEFSLK DVMVLNLTLN EILLTKIIER AQKEFVIEKS LNRIKKFWKE 1380
AQYEVIEHSS GLKLVREWDV LEQACKEDLE ELVSMKASNY YKIFEQDCLD LESKLTKLSE 1440
IQVNWVEVQF YWLDLYGILG ENLDIQNFLP LETSKFKSLT SEYKMITTRA FQLDTTIEVI 1500
HIPNFDTTLK LTIDSLKMIK SSLSTFLERQ RRQFPRFYFL GNDDLLKIIG SGKHHDQVSK 1560
FMKKMFGSIE SIIFLEDFIT GVRSVEGEVL NLNEKIELKD SIQAQEWLNI LDTEIKLSVF 1620
TQFRDCLGQL KDGTDIEVVV SKYIFQAILL SAQVMWTELV EKCLQTNQFS KYWKEVDMKI 1680
KGLLDKLNKS SDNVKKKIEA LLVEYLHFNN VIGQLKNCST KEEARLLWAK VQKFYQKNDT 1740
LDDLNSVFIS QSGYLLQYKF EYIGIPERLI YTPLLLIGFA TLTDSLHQKY GGCFFGPAGT 1800
GKTETVKAFG QNLGRVVVVF NCDDSFDYQV LSRLLVGITQ IGAWGCFDEF NRLDEKVLSA 1860
VSANIQQIQN GLQVGKSHIT LLEEETPLSP HTAVFITLNP GYNGRSELPE NLKKSFREFS 1920
MKSPQSGTIA EMILQIMGFE DSKSLASKIV HFLELLSSKC SSMNHYHFGL RTLKGVLRNC 1980
SPLISEFGEG EKTVVESLKR VILPSLGDTD ELVFKDELSK IFDSAGTPLN SKAIVQCLKD 2040
AGQRSGFSMS EEFLKKCMQF YYMQKTQQAL ILVGKAGCGK TATWKTVIDA MAIFDGHANV 2100
VYVIDTKVLT KESLYGSMLK ATLEWRDGLF TSILRRVNDD ITGTFKNSRI WVVFDSDLDP 2160
EYVEAMNSVL DDNKILTLPN GERLPIPPNF RILFETDNLD HTTPATITRC GLLWFSTDVC 2220
SISSKIDHLL NKSYEALDNK LSMFELDKLK DLISDSFDMA SLTNIFTCSN DLVHILGVRT 2280
FNKLETAVQL AVHLISSYRQ WFQNLDDKSL KDVITLLIKR SLLYALAGDS TGESQRAFIQ 2340
TINTYFGHDS QELSDYSTIV IANDKLSFSS FCSEIPSVSL EAHEVMRPDI VIPTIDTIKH 2400
EKIFYDLLNS KRGIILCGPP GSGKTMIMNN ALRNSSLYDV VGINFSKDTT TEHILSALHR 2460
HTNYVTTSKG LTLLPKSDIK NLVLFCDEIN LPKLDKYGSQ NVVLFLRQLM EKQGFWKTPE 2520
NKWVTIERIH IVGACNPPTD PGRIPMSERF TRHAAILYLG YPSGKSLSQI YEIYYKAIFK 2580
LVPEFRSYTE PFARASVHLY NECKARYSTG LQSHYLFSPR ELTRLVRGVY TAINTGPRQT 2640
LRSLIRLWAY EAWRIFADRL VGVKEKNSFE QLLYETVDKY LPNQDLGNIS STSLLFSGLL 2700
SLDFKEVNKT DLVNFIEERF KTFCDEELEV PMVIHESMVD HILRIDRALK QVQGHMMLIG 2760
ASRTGKTILT RFVAWLNGLK IVQPKIHRHS NLSDFDMILK KAISDCSLKE SRTCLIIDES 2820
NILETAFLER MNTLLANADI PDLFQGEEYD KLLNNLRNKT RSLGLLLDTE QELYDWFVGE 2880
IAKNLHVVFT ICDPTNNKSS AMISSPALFN RCIINWMGDW DTKTMSQVAN NMVDVIPMEF 2940
TDFIVPEVNK ELVFTEPIQT IRDAVVNILI HFDRNFYQKM KVGVNPRSPG YFIDGLRALV 3000
KLVTAKYQDL QENQRFVNVG LEKLNESVLK VNELNKTLSK KSTELTEKEK EARSTLDKML 3060
MEQNESERKQ EATEEIKKIL KVQEEDIRKR KEVVMKSIQD IEPTILEAQR GVKNIKKQQL 3120
TEIRSMVNPP SGVKIVMEAV CAILGYQFSN WRDIQQFIRK DDFIHNIVHY DTTLHMKPQI 3180
RKYMEEEFLS DPNFTYETIN RASKACGPLY QWVNAQINFS KVLENVDPLR QEMKRIEFES 3240
LKTKANLLAA EEMTQDLEAS IEVSKRKYSL LIRDVEAIKT EMSNVQANLD RSISLVKSLT 3300
FEKERWLNTT KQFSKTSQEL IGNCIISSIY ETYFGHLNER ERADMLVILK RLLGKFAVKY 3360
DVNYRFIDYL VTLDEKMKWL ECGLDKNDYF LENMSIVMNS QDAVPFLLDP SSHMITVISN 3420
YYGNKTVLLS FLEEGFVKRL ENAIRFGSVV IIQDGEFFDP IISRLISREF NHAGNRVTVE 3480
IGDHEVDVSG DFKLFIHSCD PSGDIPIFLR SRVRLVHFVT NKESIETRIF DITLTEENAE 3540
MQRKREDLIK LNTEYKLKLK NLEKRLLEEL NNSQGNMLEN DELMVTLNNL KKEAMNIEKK 3600
LSESEEFFPQ FDNLVEEYSI IGKHSVKIFS MLEKFGQFHW FYGISIGQFL SCFKRVFIKK 3660
SRETRAARTR VDEILWLLYQ EVYCQFSTAL DKKFKMIMAM TMFCLYKFDI ESEQYKEAVL 3720
TMIGVLSESS DGVPKLTVDT NNDLRYLWDY VTTKSYISAL NWFKNEFFVD EWNIADVVAN 3780
SENNYFTMAS ERDVDGTFKL IELAKASKES LKIIPLGSIE NLNYAQEEIS KSKIEGGWIL 3840
LQNIQMSLSW VKTYLHKHVE ETKAAEEHEK FKMFMTCHLT GDKLPAPLLQ RTDRFVYEDI 3900
PGILDTVKDL WGSQFFTGKI SGVWSVYCTF LLSWFHALIT ARTRLVPHGF SKKYYFNDCD 3960
FQFASVYLEN VLATNSTNNI PWAQVRDHIA TIVYGGKIDE EKDLEVVAKL CAHVFCGSDN 4020
LQIVPGVRIP QPLLQQSEEE ERARLTAILS NTIEPADSLS SWLQLPRESI LNYERLQAKE 4080
VASSTEQLLQ EM 4092 
Gene Ontology
 GO:0000235; C:astral microtubule; IDA:SGD.
 GO:0005938; C:cell cortex; IDA:SGD.
 GO:0005868; C:cytoplasmic dynein complex; TAS:SGD.
 GO:0005816; C:spindle pole body; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; IEA:InterPro.
 GO:0003777; F:microtubule motor activity; IDA:SGD.
 GO:0006200; P:ATP catabolic process; IEA:GOC.
 GO:0000132; P:establishment of mitotic spindle orientation; IMP:SGD.
 GO:0000741; P:karyogamy; IEA:UniProtKB-KW.
 GO:0000070; P:mitotic sister chromatid segregation; IGI:SGD.
 GO:0000022; P:mitotic spindle elongation; TAS:SGD.
 GO:0030473; P:nuclear migration along microtubule; TAS:SGD. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR011704; ATPase_dyneun-rel_AAA.
 IPR026983; DHC_fam.
 IPR024743; Dynein_HC_stalk.
 IPR024317; Dynein_heavy_chain_D4_dom.
 IPR004273; Dynein_heavy_dom.
 IPR013594; Dynein_heavy_dom-1.
 IPR013602; Dynein_heavy_dom-2.
 IPR027417; P-loop_NTPase. 
Pfam
 PF07728; AAA_5
 PF12780; AAA_8
 PF08385; DHC_N1
 PF08393; DHC_N2
 PF03028; Dynein_heavy
 PF12777; MT 
SMART
 SM00382; AAA 
PROSITE
  
PRINTS