CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-025417
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Succinyl-CoA:3-ketoacid-coenzyme A transferase 
Protein Synonyms/Alias
  
Gene Name
 Oxct1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
41VSTRHHTKFYTDPVEacetylation[1]
173QEGGSPIKYNKDGSVacetylation[2]
176GSPIKYNKDGSVAIAacetylation[3]
176GSPIKYNKDGSVAIAsuccinylation[3]
185GSVAIASKPREVREFacetylation[1, 3]
185GSVAIASKPREVREFsuccinylation[3]
418ANWMIPGKMVKGMGGacetylation[1, 2, 3, 4]
418ANWMIPGKMVKGMGGsuccinylation[3]
421MIPGKMVKGMGGAMDacetylation[1, 3, 4]
421MIPGKMVKGMGGAMDsuccinylation[3]
421MIPGKMVKGMGGAMDubiquitination[5]
434MDLVSSSKTKVVVTMacetylation[3]
434MDLVSSSKTKVVVTMsuccinylation[3]
451SAKGNAHKIMEKCTLacetylation[3]
451SAKGNAHKIMEKCTLsuccinylation[3]
455NAHKIMEKCTLPLTGacetylation[1, 2, 3, 4]
455NAHKIMEKCTLPLTGsuccinylation[3]
Reference
 [1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate (By similarity). 
Sequence Annotation
  
Keyword
 Complete proteome; Mitochondrion; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 486 AA 
Protein Sequence
MAALKLLSSG LRLGASARSS RGALHKGCVC YFSVSTRHHT KFYTDPVEAV KDIPNGATLL 60
VGGFGLCGIP ENLIGALLKT GVKDLTAVSN NAGVDNFGLG LLLRSKQIKR MISSYVGENA 120
EFERQFLSGE LEVELTPQGT LAERIRAGGA GVPAFYTSTG YGTLVQEGGS PIKYNKDGSV 180
AIASKPREVR EFNGQHFILE EAITGDFALV KAWKADRAGN VIFRKSARNF NLPMCKAAGT 240
TVVEVEEIVD IGSFAPEDIH IPKIYVHRLI KGEKYEKRIE RLSLRKEGDG KGKSGKPGGD 300
VRERIIKRAA LEFEDGMYAN LGIGIPLLAS NFISPNMTVH LQSENGVLGL GPYPLKDEAD 360
ADLINAGKET VTVLPGASFF SSDESFAMIR GGHVNLTMLG AMQVSKYGDL ANWMIPGKMV 420
KGMGGAMDLV SSSKTKVVVT MEHSAKGNAH KIMEKCTLPL TGKQCVNRII TEKVSPNLMP 480
MQQIST 486 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0008260; F:3-oxoacid CoA-transferase activity; IDA:MGI.
 GO:0046952; P:ketone body catabolic process; IMP:MGI.
 GO:0042182; P:ketone catabolic process; IMP:MGI. 
Interpro
 IPR012792; 3-oxoacid_CoA-transf_A.
 IPR012791; 3-oxoacid_CoA-transf_B.
 IPR014388; 3-oxoacid_CoA-transferase.
 IPR004165; CoA_trans_fam_I.
 IPR004164; CoA_transf_AS.
 IPR004163; CoA_transf_BS. 
Pfam
 PF01144; CoA_trans 
SMART
 SM00882; CoA_trans 
PROSITE
 PS01273; COA_TRANSF_1
 PS01274; COA_TRANSF_2 
PRINTS