CPLM-022412

Genbank Nucleotide ID

Very-long-chain enoyl-CoA reductase

Protein Synonyms/Alias

Synaptic glycoprotein SC2; Trans-2,3-enoyl-CoA reductase; TER

Homo sapiens (Human)

Position	Peptide	Type	References
2	******MKHYEVEIL	ubiquitination	[1, 2, 3, 4, 5]
12	EVEILDAKTREKLCF	ubiquitination	[1, 2, 4, 5]
16	LDAKTREKLCFLDKV	ubiquitination	[4]
22	EKLCFLDKVEPHATI	acetylation	[6]
22	EKLCFLDKVEPHATI	ubiquitination	[1, 4, 5]
33	HATIAEIKNLFTKTH	ubiquitination	[1, 2, 5]
38	EIKNLFTKTHPQWYP	ubiquitination	[1, 2, 4, 5]
57	LRLDPKGKSLKDEDV	ubiquitination	[2, 4]
60	DPKGKSLKDEDVLQK	ubiquitination	[2]
67	KDEDVLQKLPVGTTA	ubiquitination	[1, 2, 5]
116	VPFIYGHKYDFTSSR	ubiquitination	[1, 5]
224	DLRPAGSKTRKIPYP	ubiquitination	[2]
291	GKHRSYLKEFRDYPP	ubiquitination	[1, 2, 3, 4, 5, 7]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]

Functional Description

Reduces trans-2,3-stearoyl-CoA to stearoyl-CoA of long and very long chain fatty acids.

MOD_RES 22 22 N6-acetyllysine.
MOD_RES 116 116 N6-acetyllysine (By similarity).
CARBOHYD 164 164 N-linked (GlcNAc...) (Potential).
CARBOHYD 247 247 N-linked (GlcNAc...) (Potential).

3D-structure; Acetylation; Alternative splicing; Complete proteome; Disease mutation; Endoplasmic reticulum; Fatty acid biosynthesis; Fatty acid metabolism; Glycoprotein; Lipid biosynthesis; Lipid metabolism; Membrane; Mental retardation; NADP; Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0030176; C:integral to endoplasmic reticulum membrane; IDA:UniProtKB.
GO:0016627; F:oxidoreductase activity, acting on the CH-CH group of donors; IEA:InterPro.
GO:0030497; P:fatty acid elongation; IDA:UniProtKB.
GO:0035338; P:long-chain fatty-acyl-CoA biosynthetic process; TAS:Reactome.
GO:0019432; P:triglyceride biosynthetic process; TAS:Reactome.
GO:0042761; P:very long-chain fatty acid biosynthetic process; IDA:UniProtKB.

IPR001104; 3-oxo-5_a-steroid_4-DH_C.

PS50244; S5A_REDUCTASE