CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006382
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable serine/threonine-protein kinase HSL1 
Protein Synonyms/Alias
  
Gene Name
 HSL1 
Gene Synonyms/Alias
 YKL101W; YKL453 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
331LLKVQSGKYQMPSNLubiquitination[1]
356ILVIDPEKRITTQEIubiquitination[1]
747EEEEDNEKERDTQRQubiquitination[1]
1100NKINTSPKASSFTKEacetylation[2]
1100NKINTSPKASSFTKEubiquitination[1]
1111FTKEKVIKSAAYISKubiquitination[1]
1120AAYISKEKEPDNSDTubiquitination[1]
1173SRASVHRKAVSIDTMubiquitination[1]
1339VLPVVEKKENANNTEubiquitination[1]
1362PSTIKVTKDTAMESNubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
  
Sequence Annotation
 DOMAIN 81 369 Protein kinase.
 NP_BIND 87 95 ATP (By similarity).
 ACT_SITE 239 239 Proton acceptor (By similarity).
 BINDING 110 110 ATP (By similarity).
 MOD_RES 511 511 Phosphoserine.
 MOD_RES 685 685 Phosphoserine.
 MOD_RES 837 837 Phosphoserine.
 MOD_RES 866 866 Phosphoserine.
 MOD_RES 1220 1220 Phosphoserine.
 MOD_RES 1250 1250 Phosphoserine.
 MOD_RES 1284 1284 Phosphoserine.
 MOD_RES 1287 1287 Phosphoserine.
 MOD_RES 1325 1325 Phosphoserine.  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1518 AA 
Protein Sequence
MTGHVSKTSH VPKGRPSSLA KKAAKRAMAK VNSNPKRASG HLERVVQSVN DATKRLSQPD 60
STVSVATKSS KRKSRDTVGP WKLGKTLGKG SSGRVRLAKN METGQLAAIK IVPKKKAFVH 120
CSNNGTVPNS YSSSMVTSNV SSPSIASREH SNHSQTNPYG IEREIVIMKL ISHTNVMALF 180
EVWENKSELY LVLEYVDGGE LFDYLVSKGK LPEREAIHYF KQIVEGVSYC HSFNICHRDL 240
KPENLLLDKK NRRIKIADFG MAALELPNKL LKTSCGSPHY ASPEIVMGRP YHGGPSDVWS 300
CGIVLFALLT GHLPFNDDNI KKLLLKVQSG KYQMPSNLSS EARDLISKIL VIDPEKRITT 360
QEILKHPLIK KYDDLPVNKV LRKMRKDNMA RGKSNSDLHL LNNVSPSIVT LHSKGEIDES 420
ILRSLQILWH GVSRELITAK LLQKPMSEEK LFYSLLLQYK QRHSISLSSS SENKKSATES 480
SVNEPRIEYA SKTANNTGLR SENNDVKTLH SLEIHSEDTS TVNQNNAITG VNTEINAPVL 540
AQKSQFSINT LSQPESDKAE AEAVTLPPAI PIFNASSSRI FRNSYTSISS RSRRSLRLSN 600
SRLSLSASTS RETVHDNEMP LPQLPKSPSR YSLSRRAIHA SPSTKSIHKS LSRKNIAATV 660
AARRTLQNSA SKRSLYSLQS ISKRSLNLND LLVFDDPLPS KKPASENVNK SEPHSLESDS 720
DFEILCDQIL FGNALDRILE EEEDNEKERD TQRQRQNDTK SSADTFTISG VSTNKENEGP 780
EYPTKIEKNQ FNMSYKPSEN MSGLSSFPIF EKENTLSSSY LEEQKPKRAA LSDITNSFNK 840
MNKQEGMRIE KKIQREQLQK KNDRPSPLKP IQHQELRVNS LPNDQGKPSL SLDPRRNISQ 900
PVNSKVESLL QGLKFKKEPA SHWTHERGSL FMSEHVEDEK PVKASDVSIE SSYVPLTTVA 960
TSSRDPSVLA ESSTIQKPML SLPSSFLNTS MTFKNLSQIL ADDGDDKHLS VPQNQSRSVA 1020
MSHPLRKQSA KISLTPRSNL NANLSVKRNQ GSPGSYLSND LDGISDMTFA MEIPTNTFTA 1080
QAIQLMNNDT DNNKINTSPK ASSFTKEKVI KSAAYISKEK EPDNSDTNYI PDYTIPNTYD 1140
EKAINIFEDA PSDEGSLNTS SSESDSRASV HRKAVSIDTM ATTNVLTPAT NVRVSLYWNN 1200
NSSGIPRETT EEILSKLRLS PENPSNTHMQ KRFSSTRGSR DSNALGISQS LQSMFKDLEE 1260
DQDGHTSQAD ILESSMSYSK RRPSEESVNP KQRVTMLFDE EEEESKKVGG GKIKEEHTKL 1320
DNKISEESSQ LVLPVVEKKE NANNTENNYS KIPKPSTIKV TKDTAMESNT QTHTKKPILK 1380
SVQNVEVEEA PSSDKKNWFV KLFQNFSSHN NATKASKNHV TNISFDDAHM LTLNEFNKNS 1440
IDYQLKNLDH KFGRKVVEYD CKFVKGNFKF KIKITSTPNA STVITVKKRS KHSNTSSNKA 1500
FEKFNDDVER VIRNAGRS 1518 
Gene Ontology
 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0005940; C:septin ring; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004672; F:protein kinase activity; IDA:SGD.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0000078; P:cell shape checkpoint; TAS:SGD.
 GO:0000086; P:G2/M transition of mitotic cell cycle; IMP:SGD.
 GO:0046777; P:protein autophosphorylation; IMP:SGD.
 GO:0000135; P:septin checkpoint; IGI:SGD. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS