CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022857
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase RNF14 
Protein Synonyms/Alias
 Androgen receptor-associated protein 54; HFB30; RING finger protein 14; Triad2 protein 
Gene Name
 RNF14 
Gene Synonyms/Alias
 ARA54; HRIHFB2038 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
269CLNCPEPKCPSVATPubiquitination[1]
351YHGVSPCKVTAEKLMubiquitination[1]
356PCKVTAEKLMDLRNEubiquitination[1]
372LQADEANKRLLDQRYubiquitination[1, 2]
386YGKRVIQKALEEMESubiquitination[1, 2, 3, 4]
394ALEEMESKEWLEKNSubiquitination[1]
399ESKEWLEKNSKSCPCubiquitination[1]
402EWLEKNSKSCPCCGTubiquitination[1]
444LSRANPYKHFNDPGSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription. 
Sequence Annotation
 DOMAIN 11 137 RWD.
 ZN_FING 220 270 RING-type 1; atypical.
 ZN_FING 289 350 IBR-type.
 ZN_FING 404 433 RING-type 2; degenerate.
 REGION 361 474 Interaction with androgen receptor.
 MOTIF 37 45 D-box.
 MOD_RES 348 348 Phosphoserine.  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Ligase; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 474 AA 
Protein Sequence
MSSEDREAQE DELLALASIY DGDEFRKAES VQGGETRIYL DLPQNFKIFV SGNSNECLQN 60
SGFEYTICFL PPLVLNFELP PDYPSSSPPS FTLSGKWLSP TQLSALCKHL DNLWEEHRGS 120
VVLFAWMQFL KEETLAYLNI VSPFELKIGS QKKVQRRTAQ ASPNTELDFG GAAGSDVDQE 180
EIVDERAVQD VESLSNLIQE ILDFDQAQQI KCFNSKLFLC SICFCEKLGS ECMYFLECRH 240
VYCKACLKDY FEIQIRDGQV QCLNCPEPKC PSVATPGQVK ELVEAELFAR YDRLLLQSSL 300
DLMADVVYCP RPCCQLPVMQ EPGCTMGICS SCNFAFCTLC RLTYHGVSPC KVTAEKLMDL 360
RNEYLQADEA NKRLLDQRYG KRVIQKALEE MESKEWLEKN SKSCPCCGTP IEKLDGCNKM 420
TCTGCMQYFC WICMGSLSRA NPYKHFNDPG SPCFNRLFYA VDVDDDIWED EVED 474 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0019787; F:small conjugating protein ligase activity; IDA:UniProtKB.
 GO:0003713; F:transcription coactivator activity; NAS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0030521; P:androgen receptor signaling pathway; NAS:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0016567; P:protein ubiquitination; IEP:UniProtKB.
 GO:0060765; P:regulation of androgen receptor signaling pathway; IDA:UniProtKB.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:UniProtKB.
 GO:0032355; P:response to estradiol stimulus; IDA:BHF-UCL.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR006575; RWD-domain.
 IPR016135; UBQ-conjugating_enzyme/RWD.
 IPR002867; Znf_C6HC.
 IPR001841; Znf_RING.
 IPR017907; Znf_RING_CS. 
Pfam
 PF01485; IBR
 PF05773; RWD 
SMART
 SM00647; IBR
 SM00184; RING
 SM00591; RWD 
PROSITE
 PS50908; RWD
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS