CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018805
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 USP6 N-terminal-like protein 
Protein Synonyms/Alias
 Related to the N-terminus of tre; RN-tre 
Gene Name
 USP6NL 
Gene Synonyms/Alias
 KIAA0019 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10SDQDVALKLAQERAEubiquitination[1]
86KMLKGWEKYKNTEKFubiquitination[1]
88LKGWEKYKNTEKFHRubiquitination[1]
131RDLYSKLKHRARGCSubiquitination[2]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Acts as a GTPase-activating protein for RAB5A and RAB43. Involved in receptor trafficking. In complex with EPS8 inhibits internalization of EGFR. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for structural integrity of the Golgi complex. 
Sequence Annotation
 DOMAIN 100 292 Rab-GAP TBC.
 MOD_RES 391 391 Phosphoserine.
 MOD_RES 396 396 Phosphoserine.
 MOD_RES 546 546 Phosphoserine (By similarity).
 MOD_RES 582 582 Phosphotyrosine.
 MOD_RES 585 585 Phosphoserine.
 MOD_RES 642 642 Phosphoserine.
 MOD_RES 655 655 Phosphoserine.
 MOD_RES 659 659 Phosphoserine.
 MOD_RES 673 673 Phosphoserine (By similarity).
 MOD_RES 680 680 Phosphoserine.
 MOD_RES 716 716 Phosphoserine.
 MOD_RES 729 729 Phosphotyrosine.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasmic vesicle; Golgi apparatus; GTPase activation; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 828 AA 
Protein Sequence
MNSDQDVALK LAQERAEIVA KYDRGREGAE IEPWEDADYL VYKVTDRFGF LHEEELPDHN 60
VAVERQKHLE IERTTKWLKM LKGWEKYKNT EKFHRRIYKG IPLQLRGEVW ALLLEIPKMK 120
EETRDLYSKL KHRARGCSPD IRQIDLDVNR TFRDHIMFRD RYGVKQQSLF HVLAAYSIYN 180
TEVGYCQGMS QITALLLMYM NEEDAFWALV KLFSGPKHAM HGFFVQGFPK LLRFQEHHEK 240
ILNKFLSKLK QHLDSQEIYT SFYTMKWFFQ CFLDRTPFTL NLRIWDIYIF EGERVLTAMS 300
YTILKLHKKH LMKLSMEELV EFFQETLAKD FFFEDDFVIE QLQISMTELK RAKLDLPEPG 360
KEDEYPKKPL GQLPPELQSW GVHHLSNGQR SVGRPSPLAS GRRESGAPHR RHEHSPHPQS 420
RTGTPERAQP PRRKSVEEES KKLKDEADFQ RKLPSGPQDS SRQYNHAAAN QNSNATSNIR 480
KEFVPKWNKP SDVSATERTA KYTMEGKGRA AHPALAVTVP GPAEVRVSNV RPKMKALDAE 540
DGKRGSTASQ YDNVPGPELD SGASVEEALE RAYSQSPRHA LYPPSPRKHA EPSSSPSKVS 600
NKFTFKVQPP SHARYPSQLD GEARGLAHPP SYSNPPVYHG NSPKHFPTAN SSFASPQFSP 660
GTQLNPSRRP HGSTLSVSAS PEKSYSRPSP LVLPSSRIEV LPVDTGAGGY SGNSGSPKNG 720
KLIIPPVDYL PDNRTWSEVS YTYRPETQGQ SWTRDASRGN LPKYSAFQLA PFQDHGLPAV 780
SVDSPVRYKA SPAAEDASPS GYPYSGPPPP AYHYRNRDGL SIQESVLL 828 
Gene Ontology
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IEA:UniProtKB-SubCell.
 GO:0031410; C:cytoplasmic vesicle; IDA:UniProtKB.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0005097; F:Rab GTPase activator activity; IDA:UniProtKB.
 GO:0007030; P:Golgi organization; IMP:UniProtKB.
 GO:0035526; P:retrograde transport, plasma membrane to Golgi; IMP:UniProtKB. 
Interpro
 IPR000195; Rab-GTPase-TBC_dom. 
Pfam
 PF00566; RabGAP-TBC 
SMART
 SM00164; TBC 
PROSITE
 PS50086; TBC_RABGAP 
PRINTS