CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015053
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 3-isopropylmalate dehydratase large subunit 
Protein Synonyms/Alias
 Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase 
Gene Name
 leuC 
Gene Synonyms/Alias
 RPA0240 
Created Date
 July 27, 2013 
Organism
 Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) 
NCBI Taxa ID
 258594 
Lysine Modification
Position
Peptide
Type
References
336MGLTPGTKITDIKIDacetylation[1]
368AAKVAEGKTVNANVNacetylation[1]
Reference
 [1] System-wide studies of N-lysine acetylation in Rhodopseudomonas palustris reveal substrate specificity of protein acetyltransferases.
 Crosby HA, Pelletier DA, Hurst GB, Escalante-Semerena JC.
 J Biol Chem. 2012 May 4;287(19):15590-601. [PMID: 22416131] 
Functional Description
 Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate (By similarity). 
Sequence Annotation
 METAL 350 350 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 410 410 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 413 413 Iron-sulfur (4Fe-4S) (By similarity).  
Keyword
 4Fe-4S; Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Iron; Iron-sulfur; Leucine biosynthesis; Lyase; Metal-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 469 AA 
Protein Sequence
MSAKPTTLYD KIWNDHLVHE AEDGTCLLYI DRHLVHEVTS PQAFEGLRTA GRKVHAPEKT 60
LAVVDHNVPT TDRSKPNPDP ESAEQIAALA ENARDFGITY YNEFDKRQGV VHVIGPEQGF 120
TLPGTTIVCG DSHTSTHGAF GALAHGIGTS EVEHVLATQT LIQKKAKNMR VTVDGQLPDG 180
VTAKDVILAI IGEIGTAGGT GYVLEYAGDA IRSLSMEGRM TVCNMSIEGG ARAGLIAPDE 240
RAYEFLKGRP LAPKGAAWDA ALRYWDTLRS DDGAHFDHEL KLDAAALPPI VTWGTSPEDV 300
ISVTGRVPNP ADIADEAKRL SKERALAYMG LTPGTKITDI KIDRMFIGSC TNGRIEDLRA 360
AAKVAEGKTV NANVNAIIVP GSGLVKEQAE AEGLDKIFIK AGFEWREPGC SMCLAMNPDK 420
LAPEERCAST SNRNFEGRQG FKGRTHLVSP AMAAAAAIAG HFVDIRDWH 469 
Gene Ontology
 GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:HAMAP.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0009098; P:leucine biosynthetic process; IEA:HAMAP. 
Interpro
 IPR004430; 3-IsopropMal_deHydase_lsu.
 IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
 IPR015937; Acoase/IPM_deHydtase.
 IPR001030; Acoase/IPM_deHydtase_lsu_aba.
 IPR015932; Aconitase/IPMdHydase_lsu_aba_2.
 IPR018136; Aconitase_4Fe-4S_BS. 
Pfam
 PF00330; Aconitase 
SMART
  
PROSITE
 PS00450; ACONITASE_1
 PS01244; ACONITASE_2 
PRINTS
 PR00415; ACONITASE.