CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024714
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutaminase kidney isoform, mitochondrial 
Protein Synonyms/Alias
 GLS 
Gene Name
 Gls 
Gene Synonyms/Alias
 Gls1; Kiaa0838 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
135MVAAGDNKIKQGLLPacetylation[1]
135MVAAGDNKIKQGLLPsuccinylation[1]
163QEKIPVHKFITALKSacetylation[2]
169HKFITALKSTGLRTSacetylation[1, 2, 3]
169HKFITALKSTGLRTSsuccinylation[1]
169HKFITALKSTGLRTSubiquitination[4]
202SDGVMLDKDLFKKCVacetylation[5]
223LTQAFRRKFVIPDFMacetylation[2]
284RHSIGDTKVPFCLQSacetylation[2]
294FCLQSCVKPLKYAIAacetylation[2]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. 
Sequence Annotation
 REPEAT 590 619 ANK 1.
 REPEAT 624 653 ANK 2.
 BINDING 291 291 Substrate.
 BINDING 340 340 Substrate.
 BINDING 386 386 Substrate.
 BINDING 393 393 Substrate.
 BINDING 419 419 Substrate.
 BINDING 471 471 Substrate.
 BINDING 489 489 Substrate; via amide nitrogen.
 MOD_RES 316 316 N6-acetyllysine (By similarity).  
Keyword
 3D-structure; Acetylation; Alternative splicing; ANK repeat; Complete proteome; Cytoplasm; Hydrolase; Mitochondrion; Reference proteome; Repeat; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 674 AA 
Protein Sequence
MMRLRGSAML RELLLRPPAA VGAVLRRAQP LGTLCRRPRG GSRPTAGLVA AARLHPWWGG 60
GGRAKGPGAG GLSSSPSEIL QELGKGGTPP QQQQQQQQQP GASPPAAPGP KDSPGETDAF 120
GNSEGKEMVA AGDNKIKQGL LPSLEDLLFY TIAEGQEKIP VHKFITALKS TGLRTSDPRL 180
KECMDMLRLT LQTTSDGVML DKDLFKKCVQ SNIVLLTQAF RRKFVIPDFM SFTSHIDELY 240
ESAKKQSGGK VADYIPQLAK FSPDLWGVSV CTVDGQRHSI GDTKVPFCLQ SCVKPLKYAI 300
AVNDLGTEYV HRYVGKEPSG LRFNKLFLNE DDKPHNPMVN AGAIVVTSLI KQGVNNAEKF 360
DYVMQFLNKM AGNEYVGFSN ATFQSERESG DRNFAIGYYL KEKKCFPEGT DMVGILDFYF 420
QLCSIEVTCE SASVMAATLA NGGFCPITGE RVLSPEAVRN TLSLMHSCGM YDFSGQFAFH 480
VGLPAKSGVA GGILLVVPNV MGMMCWSPPL DKMGNSVKGI HFCHDLVSLC NFHNYDNLRH 540
FAKKLDPRRE GGDQRVKSVI NLLFAAYTGD VSALRRFALS AMDMEQRDYD SRTALHVAAA 600
EGHVEVVKFL LEACKVNPFP KDRWNNTPMD EALHFGHHDV FKILQEYQVQ YTPQGDSDDG 660
KGNQTVHKNL DGLL 674 
Gene Ontology
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0004359; F:glutaminase activity; IDA:UniProtKB.
 GO:0006537; P:glutamate biosynthetic process; IDA:UniProtKB.
 GO:0006543; P:glutamine catabolic process; IDA:UniProtKB.
 GO:0051289; P:protein homotetramerization; IDA:UniProtKB.
 GO:0002087; P:regulation of respiratory gaseous exchange by neurological system process; IMP:MGI.
 GO:0001967; P:suckling behavior; IMP:MGI.
 GO:0007268; P:synaptic transmission; IMP:MGI. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR012338; Beta-lactam/transpept-like.
 IPR015868; Glutaminase. 
Pfam
 PF12796; Ank_2
 PF04960; Glutaminase 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT 
PRINTS