CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018037
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glucosamine-6-phosphate isomerase 2 
Protein Synonyms/Alias
 Glucosamine-6-phosphate deaminase 2; GNPDA 2; GlcN6P deaminase 2; Glucosamine-6-phosphate isomerase SB52 
Gene Name
 GNPDA2 
Gene Synonyms/Alias
 GNP2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
28CNRIIQFKPGQDRYFubiquitination[1, 2]
51TPLGCYKKLIEYHKNubiquitination[2, 3]
67HLSFKYVKTFNMDEYubiquitination[1, 4]
124ECDAFENKIKEAGGIubiquitination[2]
126DAFENKIKEAGGIDLubiquitination[2]
160LVSRTRLKTLAMDTIubiquitination[1, 2, 5]
172DTILANAKYFDGDLSubiquitination[1, 5, 6]
273VDPLFSMKDGN****ubiquitination[1, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
 ACT_SITE 72 72 Proton acceptor; for enolization step (By
 ACT_SITE 141 141 For ring-opening step (By similarity).
 ACT_SITE 143 143 Proton acceptor; for ring-opening step
 ACT_SITE 148 148 For ring-opening step (By similarity).  
Keyword
 Alternative splicing; Carbohydrate metabolism; Coiled coil; Complete proteome; Cytoplasm; Hydrolase; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 276 AA 
Protein Sequence
MRLVILDNYD LASEWAAKYI CNRIIQFKPG QDRYFTLGLP TGSTPLGCYK KLIEYHKNGH 60
LSFKYVKTFN MDEYVGLPRN HPESYHSYMW NNFFKHIDID PNNAHILDGN AADLQAECDA 120
FENKIKEAGG IDLFVGGIGP DGHIAFNEPG SSLVSRTRLK TLAMDTILAN AKYFDGDLSK 180
VPTMALTVGV GTVMDAREVM ILITGAHKAF ALYKAIEEGV NHMWTVSAFQ QHPRTIFVCD 240
EDATLELRVK TVKYFKGLMH VHNKLVDPLF SMKDGN 276 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004342; F:glucosamine-6-phosphate deaminase activity; IEA:EC.
 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
 GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
 GO:0006044; P:N-acetylglucosamine metabolic process; IEA:InterPro. 
Interpro
 IPR006148; Glc/Gal-6P_isomerase.
 IPR004547; Glucosamine6P_isomerase.
 IPR018321; Glucosamine6P_isomerase_CS. 
Pfam
 PF01182; Glucosamine_iso 
SMART
  
PROSITE
 PS01161; GLC_GALNAC_ISOMERASE 
PRINTS