CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016902
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Coatomer subunit alpha 
Protein Synonyms/Alias
 Alpha-coat protein; Alpha-COP; Xenin; Xenopsin-related peptide; Proxenin 
Gene Name
 Copa 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
46RMCTLIDKFDEHDGPubiquitination[1]
61VRGIDFHKQQPLFVSubiquitination[1]
74VSGGDDYKIKVWNYKubiquitination[1]
170DISGLRKKNLSPGAVubiquitination[1]
238IWRMNESKAWEVDTCubiquitination[1]
271ILSNSEDKSIRVWDMubiquitination[1]
350QLDFNSSKDVAVMQLubiquitination[1]
362MQLRSGSKFPVFNMSubiquitination[1]
398YDLYTIPKDADSQNPubiquitination[1]
441SLLIKNLKNEITKKIubiquitination[1]
447LKNEITKKIQVPNCDubiquitination[1]
480TLFDVQQKRTLASVKubiquitination[1]
574PIYVTRVKGNNVYCLubiquitination[1]
598TIDPTEFKFKLALINubiquitination[1]
600DPTEFKFKLALINRKubiquitination[1]
619LHMVRNAKLVGQSIIubiquitination[1]
631SIIAYLQKKGYPEVAubiquitination[1]
727MKIAEIRKDMSGHYQubiquitination[1]
750SERVRILKNCGQKSLubiquitination[1]
825FEGSIASKGKGGALAubiquitination[1]
993NWKDAGLKNGVPAVGubiquitination[1]
1028KFEEAVEKFRSILLSubiquitination[1]
1184YRGKPVEKCPLSGACubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). 
Sequence Annotation
 REPEAT 7 37 WD 1.
 REPEAT 49 79 WD 2.
 REPEAT 91 121 WD 3.
 REPEAT 133 163 WD 4.
 REPEAT 203 233 WD 5.
 REPEAT 247 277 WD 6.
 MOD_RES 173 173 Phosphoserine (By similarity).
 MOD_RES 185 185 Phosphothreonine (By similarity).
 MOD_RES 402 402 Phosphoserine (By similarity).
 MOD_RES 1035 1035 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; Cytoplasmic vesicle; ER-Golgi transport; Golgi apparatus; Hormone; Membrane; Phosphoprotein; Protein transport; Reference proteome; Repeat; Secreted; Transport; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1224 AA 
Protein Sequence
MLTKFETKSA RVKGLSFHPK RPWILTSLHN GVIQLWDYRM CTLIDKFDEH DGPVRGIDFH 60
KQQPLFVSGG DDYKIKVWNY KLRRCLFTLL GHLDYIRTTF FHHEYPWILS ASDDQTIRVW 120
NWQSRTCVCV LTGHNHYVMC AQFHPSEDLV VSASLDQTVR VWDISGLRKK NLSPGAVESD 180
VRGITGVDLF GTTDAVVKHV LEGHDRGVNW AAFHPTMPLI VSGADDRQVK IWRMNESKAW 240
EVDTCRGHYN NVSCAVFHPR QELILSNSED KSIRVWDMSK RTGVQTFRRD HDRFWVLAAH 300
PNLNLFAAGH DGGMIVFKLE RERPAYAVHG NMLHYVKDRF LRQLDFNSSK DVAVMQLRSG 360
SKFPVFNMSY NPAENAVLLC TRASNLENST YDLYTIPKDA DSQNPDAPEG KRSSGLTAVW 420
VARNRFAVLD RMHSLLIKNL KNEITKKIQV PNCDEIFYAG TGNLLLRDAD SITLFDVQQK 480
RTLASVKISK VKYVIWSADM SHVALLAKHA IVICNRKLDA LCNIHENIRV KSGAWDESGV 540
FIYTTSNHIK YAVTTGDHGI IRTLDLPIYV TRVKGNNVYC LDRECRPRVL TIDPTEFKFK 600
LALINRKYDE VLHMVRNAKL VGQSIIAYLQ KKGYPEVALH FVKDEKTRFS LALECGNIEI 660
ALEAAKALDD KNCWEKLGEV ALLQGNHQIV EMCYQRTKNF DKLSFLYLIT GNLEKLRKMM 720
KIAEIRKDMS GHYQNALYLG DVSERVRILK NCGQKSLAYL SAATHGLDEE AESLKETFDP 780
EKETIPDIDP NAKLLQPPAP IMPLDTNWPL LTVSKGFFEG SIASKGKGGA LAADIDIDTV 840
GTEGWGEDAE LQLDEDGFVE APEGLGEDVL GKGQEEGGGW DVEEDLELPP ELDVPSGVSG 900
SAEDGFFVPP TKGTSPTQIW CNNSQLPVDH ILAGSFETAM RLLHDQVGVI QFGPYKQLFL 960
QTYARGRTTY QALPCLPSMY SYPNRNWKDA GLKNGVPAVG LKLNDLIQRL QLCYQLTTVG 1020
KFEEAVEKFR SILLSVPLLV VDNKQEIAEA QQLITICREY IVGLCMEIER KKLPKETLDQ 1080
QKRICEMAAY FTHSNLQPVH MILVLRTALN LFFKLKNFKT AATFARRLLE LGPKPEVAQQ 1140
TRKILSACEK NPTDACQLNY DMHNPFDICA ASYRPIYRGK PVEKCPLSGA CYSPEFKGQI 1200
CRVTTVTEIG KDVIGLRISP LQFR 1224 
Gene Ontology
 GO:0030126; C:COPI vesicle coat; IEA:InterPro.
 GO:0005615; C:extracellular space; IEA:Compara.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0030157; P:pancreatic juice secretion; IEA:Compara.
 GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW. 
Interpro
 IPR016391; Coatomer_asu.
 IPR010714; Coatomer_asu_C.
 IPR006692; Coatomer_WD-assoc_reg.
 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF04053; Coatomer_WDAD
 PF06957; COPI_C
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.