CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020400
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Oxysterol-binding protein-related protein 8 
Protein Synonyms/Alias
 ORP-8; OSBP-related protein 8 
Gene Name
 OSBPL8 
Gene Synonyms/Alias
 KIAA1451; ORP8; OSBP10 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
183LLIYKTQKNGQWVGTacetylation[1]
224EQSIWAVKGPKGEAVubiquitination[2]
227IWAVKGPKGEAVGSIubiquitination[3]
271LKCSSLLKRTMIREGubiquitination[3]
664WNPTPDIKQWRLIRHubiquitination[3]
685QGDFESEKLWQRVTRubiquitination[3]
697VTRAINAKDQTEATQubiquitination[3]
816GSEAQSVKPSTRRKKubiquitination[4]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
  
Sequence Annotation
 DOMAIN 148 265 PH.
 MOD_RES 65 65 Phosphoserine.
 MOD_RES 68 68 Phosphoserine.
 MOD_RES 314 314 Phosphoserine.
 MOD_RES 328 328 Phosphoserine.
 MOD_RES 342 342 Phosphoserine.
 MOD_RES 807 807 Phosphoserine.
 MOD_RES 808 808 Phosphoserine.
 MOD_RES 810 810 Phosphoserine.
 MOD_RES 814 814 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Lipid transport; Lipid-binding; Phosphoprotein; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 889 AA 
Protein Sequence
MEGGLADGEP DRTSLLGDSK DVLGPSTVVA NSDESQLLTP GKMSQRQGKE AYPTPTKDLH 60
QPSLSPASPH SQGFERGKED ISQNKDESSL SMSKSKSESK LYNGSEKDSS TSSKLTKKES 120
LKVQKKNYRE EKKRATKELL STITDPSVIV MADWLKIRGT LKSWTKLWCV LKPGVLLIYK 180
TQKNGQWVGT VLLNACEIIE RPSKKDGFCF KLFHPLEQSI WAVKGPKGEA VGSITQPLPS 240
SYLIIRATSE SDGRCWMDAL ELALKCSSLL KRTMIREGKE HDLSVSSDST HVTFYGLLRA 300
NNLHSGDNFQ LNDSEIERQH FKDQDMYSDK SDKENDQEHD ESDNEVMGKS EESDTDTSER 360
QDDSYIEPEP VEPLKETTYT EQSHEELGEA GEASQTETVS EENKSLIWTL LKQVRPGMDL 420
SKVVLPTFIL EPRSFLDKLS DYYYHADFLS EAALEENPYF RLKKVVKWYL SGFYKKPKGL 480
KKPYNPILGE TFRCLWIHPR TNSKTFYIAE QVSHHPPISA FYVSNRKDGF CLSGSILAKS 540
KFYGNSLSAI LEGEARLTFL NRGEDYVMTM PYAHCKGILY GTMTLELGGT VNITCQKTGY 600
SAILEFKLKP FLGSSDCVNQ ISGKLKLGKE VLATLEGHWD SEVFITDKKT DNSEVFWNPT 660
PDIKQWRLIR HTVKFEEQGD FESEKLWQRV TRAINAKDQT EATQEKYVLE EAQRQAARDR 720
KTKNEEWSCK LFELDPLTGE WHYKFADTRP WDPLNDMIQF EKDGVIQTKV KHRTPMVSVP 780
KMKHKPTRQQ KKVAKGYSSP EPDIQDSSGS EAQSVKPSTR RKKGIELGDI QSSIESIKQT 840
QEEIKRNIMA LRNHLVSSTP ATDYFLQQKD YFIIFLLILL QVIINFMFK 889 
Gene Ontology
 GO:0015485; F:cholesterol binding; IDA:BHF-UCL.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0045444; P:fat cell differentiation; IDA:BHF-UCL.
 GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
 GO:0010891; P:negative regulation of sequestering of triglyceride; IDA:BHF-UCL. 
Interpro
 IPR000648; Oxysterol-bd.
 IPR018494; Oxysterol-bd_CS.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology. 
Pfam
 PF01237; Oxysterol_BP
 PF00169; PH 
SMART
 SM00233; PH 
PROSITE
 PS01013; OSBP
 PS50003; PH_DOMAIN 
PRINTS