| Tag | Content |
|---|
| CPLM ID | CPLM-007065 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | 25S rRNA (adenine(645)-N(1))-methyltransferase |
Protein Synonyms/Alias | Ribosomal RNA-processing protein 8 |
Gene Name | RRP8 |
Gene Synonyms/Alias | YDR083W; D4461 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 68 | PSEGSVAKDIPKKNQ | acetylation | [1] | | 359 | QKLERRQKFIEVETE | acetylation | [1] | | 381 | RRKIAEGKWLLKPCI | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(1) position of adenine 645 in 25S rRNA. N(1)-methyladenine(645) in 25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis. Required for efficient pre-rRNA cleavage at site A2. Also involved in telomere length regulation and maintenance. |
Sequence Annotation | BINDING 156 156 S-adenosyl-L-methionine (By similarity).
BINDING 207 207 S-adenosyl-L-methionine; via carbonyl
BINDING 242 242 S-adenosyl-L-methionine (By similarity).
BINDING 254 254 S-adenosyl-L-methionine (By similarity).
BINDING 271 271 S-adenosyl-L-methionine; via carbonyl
MOD_RES 62 62 Phosphoserine. |
Keyword | Chromosome; Coiled coil; Complete proteome; Methyltransferase; Nucleus; Phosphoprotein; Reference proteome; rRNA processing; S-adenosyl-L-methionine; Telomere; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 392 AA |
Protein Sequence | MALFNVEGWS IKTKTVAFDN KTNKSSKDKK KNNRKNGKLT REQKLKEETE AELKEQVEDI 60
PSEGSVAKDI PKKNQEKSDQ NETSKKRKHD EEAPLMQVKE NIEKPTKKQL TPLQQKMMAK 120
LTGSRFRWIN EQLYTISSDE ALKLIKEQPQ LFDEYHDGFR SQVQAWPENP VDVFVDQIRY 180
RCMKPVNAPG GLPGLKDSKE IVIADMGCGE AQLALEINNF FKNYNKKAKK YLKRRHKVHS 240
FDLKKANERI TVADIRNVPL PDESCTIVVF CLALMGTNFL DFIKEAYRIL APRGELWIAE 300
IKSRFSDGKG NEFVDALKLM GFFHKKTFDE NKMFTRFEFF KPPAEIIEER RQKLERRQKF 360
IEVETEKEEL EKKRRKIAEG KWLLKPCIYK RR 392 |
Gene Ontology | GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell. GO:0005730; C:nucleolus; IDA:SGD. GO:0016433; F:rRNA (adenine) methyltransferase activity; IMP:SGD. GO:0042273; P:ribosomal large subunit biogenesis; IGI:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |