UniProt Accession

 TRNT1_HUMAN; Q96Q11; A8K2Z6; B7WP13; C9JKA2; Q8ND57; Q9BS97; Q9Y362 

Genbank Protein ID

 AB063105; AF151805; AK290411; AL834397; AC024060; CH471055; BC005184; BC012537 

Genbank Nucleotide ID

 BAB70662.1; AAD34042.1; BAF83100.1; CAD39059.1; EAW63886.1; AAH05184.1; AAH12537.1 

Protein Name

 CCA tRNA nucleotidyltransferase 1, mitochondrial 

Protein Synonyms/Alias

 Mitochondrial tRNA nucleotidyl transferase, CCA-adding; mt CCA-adding enzyme; mt tRNA CCA-diphosphorylase; mt tRNA CCA-pyrophosphorylase; mt tRNA adenylyltransferase 

Gene Name

 TRNT1 

Gene Synonyms/Alias

 CGI-47 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
291	NVDGFSPKPVTLLAS	ubiquitination	[1, 2, 3, 4]
402	KVGISSGKEIGALLQ	acetylation	[4, 5, 6]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Isoform 1: Adds and repairs the conserved 3'-CCA sequence necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates. 

Sequence Annotation

 ACT_SITE 77 77 By similarity.
 ACT_SITE 79 79 By similarity.
 ACT_SITE 121 121 By similarity.
 MOD_RES 400 400 Phosphoserine.
 MOD_RES 402 402 N6-acetyllysine.
 DISULFID 373 373 Interchain.  

Keyword

 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Disulfide bond; Magnesium; Mitochondrion; Nucleotide-binding; Nucleotidyltransferase; Phosphoprotein; Polymorphism; Reference proteome; RNA-binding; Transferase; Transit peptide; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 434 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:HPA.
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0005524; F:ATP binding; TAS:UniProtKB.
 GO:0052929; F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity; IEA:EC.
 GO:0052928; F:CTP:3'-cytidine-tRNA cytidylyltransferase activity; IEA:EC.
 GO:0052927; F:CTP:tRNA cytidylyltransferase activity; IEA:EC.
 GO:0004810; F:tRNA adenylyltransferase activity; IDA:UniProtKB.
 GO:0000049; F:tRNA binding; IDA:UniProtKB.
 GO:0009022; F:tRNA nucleotidyltransferase activity; IEA:InterPro.
 GO:0006626; P:protein targeting to mitochondrion; TAS:UniProtKB.
 GO:0042780; P:tRNA 3'-end processing; IDA:UniProtKB.
 GO:0001680; P:tRNA 3'-terminal CCA addition; IEA:EC. 

Interpro

 IPR002646; PolA_pol_head_dom.
 IPR026973; Trnt1. 

Pfam

 PF01743; PolyA_pol 

SMART

  

PROSITE

  

PRINTS