CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001659
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein Wiz 
Protein Synonyms/Alias
 Widely-interspaced zinc finger-containing protein; Zinc finger protein 803 
Gene Name
 WIZ 
Gene Synonyms/Alias
 ZNF803 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
1523ASPPGTVKAEEHQRQsumoylation[1]
1597VGNIYTLKCRFCEVEubiquitination[2, 3, 4, 5, 6, 7]
1630ILEMNFSKADPPPEEubiquitination[8]
Reference
 [1] A novel proteomics approach to identify SUMOylated proteins and their modification sites in human cells.
 Galisson F, Mahrouche L, Courcelles M, Bonneil E, Meloche S, Chelbi-Alix MK, Thibault P.
 Mol Cell Proteomics. 2011 Feb;10(2):M110.004796. [PMID: 21098080]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
 May link EHMT1 and EHMT2 histone methyltransferases to the CTBP corepressor machinery. May be involved in EHMT1-EHMT2 heterodimer formation and stabilization (By similarity). 
Sequence Annotation
 ZN_FING 267 289 C2H2-type 1.
 ZN_FING 304 326 C2H2-type 2.
 ZN_FING 353 375 C2H2-type 3.
 ZN_FING 416 439 C2H2-type 4.
 ZN_FING 701 723 C2H2-type 5.
 ZN_FING 769 791 C2H2-type 6.
 ZN_FING 870 892 C2H2-type 7.
 ZN_FING 1043 1065 C2H2-type 8.
 ZN_FING 1227 1249 C2H2-type 9.
 ZN_FING 1397 1419 C2H2-type 10.
 ZN_FING 1596 1622 C2H2-type 11.
 REGION 1030 1034 Interaction with CTBP1 and CTBP2 1 (By
 REGION 1214 1218 Interaction with CTBP1 and CTBP2 2 (By
 MOD_RES 996 996 Phosphoserine.
 MOD_RES 998 998 Phosphothreonine.
 MOD_RES 1006 1006 Phosphoserine.
 MOD_RES 1012 1012 Phosphoserine.
 MOD_RES 1017 1017 Phosphoserine.
 MOD_RES 1079 1079 Phosphoserine.
 MOD_RES 1127 1127 Phosphoserine.
 MOD_RES 1134 1134 Phosphoserine.
 MOD_RES 1146 1146 Phosphoserine.
 MOD_RES 1151 1151 Phosphoserine.
 MOD_RES 1162 1162 N6,N6,N6-trimethyllysine; by EHMT2;
 MOD_RES 1162 1162 N6,N6-dimethyllysine; by EHMT2;
 MOD_RES 1263 1263 Phosphoserine.
 MOD_RES 1309 1309 Phosphoserine.
 MOD_RES 1314 1314 Phosphoserine.
 MOD_RES 1517 1517 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Metal-binding; Methylation; Nucleus; Phosphoprotein; Reference proteome; Repeat; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1651 AA 
Protein Sequence
MEGSLAGSLA APDRPQGPER LPGPAPRENI EGGAEAAEGE GGIFRSTRYL PVTKEGPRDI 60
LDGRGGISGT PDGRGPWEHP LVQEAGEGIL SERRFEDSVI VRTMKPHAEL EGSRRFLHHR 120
GEPRLLEKHA QGRPRFDWLQ DEDEQGSPQD AGLHLDLPAQ PPPLAPFRRV FVPVEDTPKT 180
LDMAVVGGRE DLEDLEGLAQ PSEWGLPTSA SEVATQTWTV NSEASVERLQ PLLPPIRTGP 240
YLCELLEEVA EGVASPDEDE DEEPAVFPCI ECSIYFKQKE HLLEHMSQHR RAPGQEPPAD 300
LAPLACGECG WAFADPTALE QHRQLHQASR EKIIEEIQKL KQVPGDEGRE ARLQCPKCVF 360
GTNSSRAYVQ HAKLHMREPP GQTTKEPFGG SSGAGSPSPE ASALLYQPYG AAVGLSACVF 420
CGFPAPSESL LREHVRLVHA HPHWEEDGEA YEEDPASQPG TSQDAHACFP DTAVDYFGKA 480
EPSLAPMWRE NPAGYDPSLA FGPGCQQLSI RDFPLSKPLL HGTGQRPLGR LAFPSTLAST 540
PYSLQLGRNK STVHPQGLGE RRRPWSEEEE EEEEEEDVVL TSEMDFSPEN GVFSPLATPS 600
LIPQAALELK QAFREALQAV EATQGQQQQL RGMVPIVLVA KLGPQVMAAA RVPPRLQPEE 660
LGLAGAHPLD FLLLDAPLGG PLGLDTLLDG DPAMALKHEE RKCPYCPDRF HNGIGLANHV 720
RGHLNRVGVS YNVRHFISAE EVKAIERRFS FQKKKKKVAN FDPGTFSLMR CDFCGAGFDT 780
RAGLSSHARA HLRDFGITNW ELTVSPINIL QELLATSAAE QPPSPLGREP GGPPGSFLTS 840
RRPRLPLTVP FPPTWAEDPG PAYGDAQSLT TCEVCGACFE TRKGLSSHAR SHLRQLGVAE 900
SESSGAPIDL LYELVKQKGL PDAHLGLPPG LAKKSSSLKE VVAGAPRPGL LSLAKPLDAP 960
AVNKAIKSPP GFSAKGLGHP PSSPLLKKTP LALAGSPTPK NPEDKSPQLS LSPRPASPKA 1020
QWPQSEDEGP LNLTSGPEPA RDIRCEFCGE FFENRKGLSS HARSHLRQMG VTEWYVNGSP 1080
IDTLREILKR RTQSRPGGPP NPPGPSPKAL AKMMGGAGPG SSLEARSPSD LHISPLAKKL 1140
PPPPGSPLGH SPTASPPPTA RKMFPGLAAP SLPKKLKPEQ IRVEIKREML PGALHGELHP 1200
SEGPWGAPRE DMTPLNLSSR AEPVRDIRCE FCGEFFENRK GLSSHARSHL RQMGVTEWSV 1260
NGSPIDTLRE ILKKKSKPCL IKKEPPAGDL APALAEDGPP TVAPGPVQSP LPLSPLAGRP 1320
GKPGAGPAQV PRELSLTPIT GAKPSATGYL GSVAAKRPLQ EDRLLPAEVK AKTYIQTELP 1380
FKAKTLHEKT SHSSTEACCE LCGLYFENRK ALASHARAHL RQFGVTEWCV NGSPIETLSE 1440
WIKHRPQKVG AYRSYIQGGR PFTKKFRSAG HGRDSDKRPS LGLAPGGLAV VGRSAGGEPG 1500
PEAGRAADGG ERPLAASPPG TVKAEEHQRQ NINKFERRQA RPPDASAARG GEDTNDLQQK 1560
LEEVRQPPPR VRPVPSLVPR PPQTSLVKFV GNIYTLKCRF CEVEFQGPLS IQEEWVRHLQ 1620
RHILEMNFSK ADPPPEESQA PQAQTAAAEA P 1651 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS