CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020720
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Poly(A) RNA polymerase, mitochondrial 
Protein Synonyms/Alias
 PAP; PAP-associated domain-containing protein 1; Polynucleotide adenylyltransferase 
Gene Name
 Mtpap 
Gene Synonyms/Alias
 Papd1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
89CPNNINEKKFLKYLSacetylation[1, 2]
89CPNNINEKKFLKYLSsuccinylation[2]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA (By similarity). 
Sequence Annotation
 DOMAIN 441 486 PAP-associated.
 NP_BIND 107 109 ATP (Potential).
 NP_BIND 244 245 ATP (Potential).
 METAL 246 246 Magnesium or manganese; catalytic
 METAL 248 248 Magnesium or manganese; catalytic
 MOD_RES 90 90 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; ATP-binding; Complete proteome; Cytoplasm; Magnesium; Manganese; Metal-binding; Mitochondrion; mRNA processing; Nucleotide-binding; Reference proteome; RNA-binding; Transcription; Transferase; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 585 AA 
Protein Sequence
MAARGVGLLT RLPVCSQRRN RIPRSISRLL SCPGTIAASI GSEEQSSVVA ETGIEDKTLQ 60
KKFSEVQKER REQAQRTVLI HCPNNINEKK FLKYLSQHGP VNNHFFYESF GLFAVVEFCQ 120
KDSIKSLQNG THTPTQSTEA AIPFKSRFLN LRLKNPSSQV SGQPFVQTTN QSPPSSKKLF 180
ELLSYAESIE EQLNTLLKAF QLTEENIRLR HLTCSLIEDI AAAYFPSCVI RPFGSSVNTF 240
GKLGCDLDMF LDLDETGKLD VHKNTGNFFM EFQVKNVPSE RIATQKILSV IGECLDNFGP 300
GCVGVQKILN ARCPLVRFSH QGSGFQCDLT ANNSIALKSS ELLYIYGSLD SRVRALVFSV 360
RCWARAHSLT SSIPGAWITN FSLTVMVIFF LQRRSPPILP TLDSLKSIAD AEDRCILEGN 420
NCTFVQDVNK IQPSGNTETL ELLIKEFFEY FGNFAFNKNS INIRQGREQN KPDSSPLYIQ 480
NPFETSLNIS KNVSQSQLQK FVELARDSAW ILEQEDKNQP FSSSRQPWGL AALLLPPGSG 540
HTSLSRKKKK KPMSEKVKGL LASIKSNSPD SSTDTSGKRT ISTQA 585 
Gene Ontology
 GO:0005794; C:Golgi apparatus; IEA:Compara.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0005524; F:ATP binding; ISS:UniProtKB.
 GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
 GO:0030145; F:manganese ion binding; ISS:UniProtKB.
 GO:0004652; F:polynucleotide adenylyltransferase activity; ISS:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0002134; F:UTP binding; ISS:UniProtKB.
 GO:0071044; P:histone mRNA catabolic process; ISS:UniProtKB.
 GO:0006378; P:mRNA polyadenylation; ISS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR002058; PAP_assoc. 
Pfam
 PF03828; PAP_assoc 
SMART
  
PROSITE
  
PRINTS