CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000257
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein 195 
Protein Synonyms/Alias
  
Gene Name
 ZNF195 
Gene Synonyms/Alias
 ZNFP104 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
223FQYNKYVKIFDNFSNubiquitination[1, 2]
383LAGEKLSKCETWYKGubiquitination[3]
408QRNEIGGKPFKCEECacetylation[4]
408QRNEIGGKPFKCEECubiquitination[3]
411EIGGKPFKCEECDSIubiquitination[3]
427KWFSDLTKHKRIHTGubiquitination[3]
473YQCEECGKVFRTCSSubiquitination[3, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 May be involved in transcriptional regulation. 
Sequence Annotation
 DOMAIN 4 75 KRAB.
 ZN_FING 244 266 C2H2-type 1.
 ZN_FING 272 294 C2H2-type 2; degenerate.
 ZN_FING 410 432 C2H2-type 3.
 ZN_FING 438 460 C2H2-type 4.
 ZN_FING 466 488 C2H2-type 5.
 ZN_FING 494 516 C2H2-type 6.
 ZN_FING 522 544 C2H2-type 7.
 ZN_FING 550 572 C2H2-type 8.
 ZN_FING 578 600 C2H2-type 9.
 ZN_FING 606 628 C2H2-type 10.
 REGION 76 243 Spacer.
 MOD_RES 408 408 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 629 AA 
Protein Sequence
MTLLTFRDVA IEFSLEEWKC LDLAQQNLYR DVMLENYRNL FSVGLTVCKP GLITCLEQRK 60
EPWNVKRQEA ADGHPEMGFH HATQACLELL GSSDLPASAS QSAGITGVNH RAQPGLNVSV 120
DKFTALCSPG VLQTVKWFLE FRCIFSLAMS SHFTQDLLPE QGIQDAFPKR ILRGYGNCGL 180
DNLYLRKDWE SLDECKLQKD YNGLNQCSST THSKIFQYNK YVKIFDNFSN LHRRNISNTG 240
EKPFKCQECG KSFQMLSFLT EHQKIHTGKK FQKCGECGKT FIQCSHFTEP ENIDTGEKPY 300
KCQECNNVIK TCSVLTKNRI YAGGEHYRCE EFGKVFNQCS HLTEHEHGTE EKPCKYEECS 360
SVFISCSSLS NQQMILAGEK LSKCETWYKG FNHSPNPSKH QRNEIGGKPF KCEECDSIFK 420
WFSDLTKHKR IHTGEKPYKC DECGKAYTQS SHLSEHRRIH TGEKPYQCEE CGKVFRTCSS 480
LSNHKRTHSE EKPYTCEECG NIFKQLSDLT KHKKTHTGEK PYKCDECGKN FTQSSNLIVH 540
KRIHTGEKPY KCEECGRVFM WFSDITKHKK THTGEKPYKC DECGKNFTQS SNLIVHKRIH 600
TGEKPYKCEK CGKAFTQFSH LTVHESIHT 629 
Gene Ontology
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0003677; F:DNA binding; NAS:UniProtKB.
 GO:0008270; F:zinc ion binding; NAS:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; NAS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001909; Krueppel-associated_box.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF01352; KRAB 
SMART
 SM00349; KRAB
 SM00355; ZnF_C2H2 
PROSITE
 PS50805; KRAB
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS