CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002826
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamate synthase [NADPH] large chain 
Protein Synonyms/Alias
 Glutamate synthase subunit alpha; GLTS alpha chain; NADPH-GOGAT 
Gene Name
 gltB 
Gene Synonyms/Alias
 aspB; b3212; JW3179 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
61GCGLLLQKPDRFFRIacetylation[1]
79ERGWRLAKNYAVGMLacetylation[1]
90VGMLFLNKDPELAAAacetylation[1]
172EKRLEADKDFYVCSLacetylation[1]
365PARYVITKDKLITCAacetylation[1]
417AETDDDLKSRHPYKEacetylation[1]
423LKSRHPYKEWMEKNVacetylation[1]
428PYKEWMEKNVRRLVPacetylation[1]
459DTLASYQKQFNYSAEacetylation[1]
553QAHRLSFKSPILLYSacetylation[1]
563ILLYSDFKQLTTMKEacetylation[1]
569FKQLTTMKEEHYRADacetylation[1]
594TTLEATVKELCDKAEacetylation[1]
599TVKELCDKAEKMVRSacetylation[1]
620LSDRNIAKDRLPVPAacetylation[1]
693VDTHAIAKDYRTVMLacetylation[1]
708NYRNGINKGLYKIMSacetylation[1]
712GINKGLYKIMSKMGIacetylation[1]
769QDLLNLSKRAWLARKacetylation[1]
776KRAWLARKPISQGGLacetylation[1]
785ISQGGLLKYVHGGEYacetylation[1]
820SDYQEYAKLVNERPAacetylation[1]
856EPASELFKRFDTAAMacetylation[1]
931NADVIQIKVAQGAKPacetylation[1]
948GGQLPGDKVTPYIAKacetylation[1]
955KVTPYIAKLRYSVPGacetylation[1]
993DLKQVNPKAMISVKLacetylation[1]
1123GVATQDDKLRKNHYHacetylation[1]
1170IGRTDLLKELDGFTAacetylation[1]
1186QQKLALSKLLETAEPacetylation[1]
1197TAEPHPGKALYCTENacetylation[1]
1222AQLLQQAKPFVDERQacetylation[1]
1231FVDERQSKTFWFDIRacetylation[1]
1456NWSTFATKFALVKPKacetylation[1]
1461ATKFALVKPKSSDVKacetylation[1]
1468KPKSSDVKALLGHRSacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
  
Sequence Annotation
 DOMAIN 12 402 Glutamine amidotransferase type-2.
 NP_BIND 1049 1101 FMN (By similarity).
 ACT_SITE 12 12 For GATase activity (By similarity).
 METAL 1102 1102 Iron-sulfur (3Fe-4S) (By similarity).
 METAL 1108 1108 Iron-sulfur (3Fe-4S) (By similarity).
 METAL 1113 1113 Iron-sulfur (3Fe-4S) (By similarity).  
Keyword
 3Fe-4S; Amino-acid biosynthesis; Complete proteome; Direct protein sequencing; FAD; Flavoprotein; FMN; Glutamate biosynthesis; Glutamine amidotransferase; Iron; Iron-sulfur; Metal-binding; NADP; Oxidoreductase; Reference proteome; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1486 AA 
Protein Sequence
MLYDKSLERD NCGFGLIAHI EGEPSHKVVR TAIHALARMQ HRGAILADGK TGDGCGLLLQ 60
KPDRFFRIVA QERGWRLAKN YAVGMLFLNK DPELAAAARR IVEEELQRET LSIVGWRDVP 120
TNEGVLGEIA LSSLPRIEQI FVNAPAGWRP RDMERRLFIA RRRIEKRLEA DKDFYVCSLS 180
NLVNIYKGLC MPTDLPRFYL DLADLRLESA ICLFHQRFST NTVPRWPLAQ PFRYLAHNGE 240
INTITGNRQW ARARTYKFQT PLIPDLHDAA PFVNETGSDS SSMDNMLELL LAGGMDIIRA 300
MRLLVPPAWQ NNPDMDPELR AFFDFNSMHM EPWDGPAGIV MSDGRFAACN LDRNGLRPAR 360
YVITKDKLIT CASEVGIWDY QPDEVVEKGR VGPGELMVID TRSGRILHSA ETDDDLKSRH 420
PYKEWMEKNV RRLVPFEDLP DEEVGSRELD DDTLASYQKQ FNYSAEELDS VIRVLGENGQ 480
EAVGSMGDDT PFAVLSSQPR IIYDYFRQQF AQVTNPPIDP LREAHVMSLA TSIGREMNVF 540
CEAEGQAHRL SFKSPILLYS DFKQLTTMKE EHYRADTLDI TFDVTKTTLE ATVKELCDKA 600
EKMVRSGTVL LVLSDRNIAK DRLPVPAPMA VGAIQTRLVD QSLRCDANII VETASARDPH 660
HFAVLLGFGA TAIYPYLAYE TLGRLVDTHA IAKDYRTVML NYRNGINKGL YKIMSKMGIS 720
TIASYRCSKL FEAVGLHDDV VGLCFQGAVS RIGGASFEDF QQDLLNLSKR AWLARKPISQ 780
GGLLKYVHGG EYHAYNPDVV RTLQQAVQSG EYSDYQEYAK LVNERPATTL RDLLAITPGE 840
NAVNIADVEP ASELFKRFDT AAMSIGALSP EAHEALAEAM NSIGGNSNSG EGGEDPARYG 900
TNKVSRIKQV ASGRFGVTPA YLVNADVIQI KVAQGAKPGE GGQLPGDKVT PYIAKLRYSV 960
PGVTLISPPP HHDIYSIEDL AQLIFDLKQV NPKAMISVKL VSEPGVGTIA TGVAKAYADL 1020
ITIAGYDGGT GASPLSSVKY AGCPWELGLV ETQQALVANG LRHKIRLQVD GGLKTGVDII 1080
KAAILGAESF GFGTGPMVAL GCKYLRICHL NNCATGVATQ DDKLRKNHYH GLPFKVTNYF 1140
EFIARETREL MAQLGVTRLV DLIGRTDLLK ELDGFTAKQQ KLALSKLLET AEPHPGKALY 1200
CTENNPPFDN GLLNAQLLQQ AKPFVDERQS KTFWFDIRNT DRSVGASLSG YIAQTHGDQG 1260
LAADPIKAYF NGTAGQSFGV WNAGGVELYL TGDANDYVGK GMAGGLIAIR PPVGSAFRSH 1320
EASIIGNTCL YGATGGRLYA AGRAGERFGV RNSGAITVVE GIGDNGCEYM TGGIVCILGK 1380
TGVNFGAGMT GGFAYVLDES GDFRKRVNPE LVEVLSVDAL AIHEEHLRGL ITEHVQHTGS 1440
QRGEEILANW STFATKFALV KPKSSDVKAL LGHRSRSAAE LRVQAQ 1486 
Gene Ontology
 GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0004355; F:glutamate synthase (NADPH) activity; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006537; P:glutamate biosynthetic process; IDA:EcoCyc.
 GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
 GO:0097054; P:L-glutamate biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR013785; Aldolase_TIM.
 IPR017932; GATase_2_dom.
 IPR000583; GATase_dom.
 IPR002489; Glu_synth_asu_C.
 IPR002932; Glu_synth_centr_C.
 IPR006982; Glu_synth_centr_N. 
Pfam
 PF00310; GATase_2
 PF04898; Glu_syn_central
 PF01645; Glu_synthase
 PF01493; GXGXG 
SMART
  
PROSITE
 PS51278; GATASE_TYPE_2 
PRINTS