UniProt Accession

 RS4B_YEAST; P0CX36; D3DLF2; P05753 

Genbank Protein ID

 M64294; U00030; BK006934 

Genbank Nucleotide ID

 AAA35012.1; AAB68372.1; DAA06896.1 

Protein Name

 40S ribosomal protein S4-B 

Protein Synonyms/Alias

 RP5; S7; YS6 

Gene Name

 RPS4B 

Gene Synonyms/Alias

 RPS7A; YHR203C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
22	PHHWLLDKLSGCYAP	ubiquitination	[1]
37	RPSAGPHKLRESLPL	ubiquitination	[1]
62	ALNGREVKAILMQRH	ubiquitination	[1]
134	KKVQLGKKGVPYVVT	ubiquitination	[1]
155	RYPDPNIKVNDTVKI	ubiquitination	[1]
161	IKVNDTVKIDLASGK	ubiquitination	[1]
168	KIDLASGKITDFIKF	ubiquitination	[1, 2, 3]
174	GKITDFIKFDAGKLV	ubiquitination	[1]
179	FIKFDAGKLVYVTGG	ubiquitination	[1]
211	GFDLVHIKDSLDNTF	ubiquitination	[1]
233	FVIGEQGKPYISLPK	ubiquitination	[1]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

  

Sequence Annotation

 DOMAIN 42 105 S4 RNA-binding.
 MOD_RES 32 32 Phosphoserine.
 MOD_RES 115 115 Phosphothreonine.
 MOD_RES 247 247 Phosphoserine.
 CROSSLNK 168 168 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; Isopeptide bond; Phosphoprotein; Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 261 AA 

Protein Sequence

Gene Ontology

 GO:0030686; C:90S preribosome; IDA:SGD.
 GO:0022627; C:cytosolic small ribosomal subunit; IDA:SGD.
 GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
 GO:0003735; F:structural constituent of ribosome; IDA:SGD.
 GO:0002181; P:cytoplasmic translation; IC:SGD. 

Interpro

 IPR005824; KOW.
 IPR000876; Ribosomal_S4e.
 IPR013845; Ribosomal_S4e_central_region.
 IPR013843; Ribosomal_S4e_N.
 IPR018199; Ribosomal_S4e_N_CS.
 IPR002942; S4_RNA-bd. 

Pfam

 PF00467; KOW
 PF00900; Ribosomal_S4e
 PF08071; RS4NT
 PF01479; S4 

SMART

 SM00739; KOW
 SM00363; S4 

PROSITE

 PS00528; RIBOSOMAL_S4E
 PS50889; S4 

PRINTS