UniProt Accession

 ALDOA_RAT; P05065; Q63038 

Genbank Protein ID

 M12919; M14420; X04261; X04262; X04263; X04264; BC064440; M28282 

Genbank Nucleotide ID

 AAA40714.1; AAA40715.1; CAA27815.1; CAA27815.1; CAA27815.1; CAA27815.1; AAH64440.1; AAA40720.1 

Protein Name

 Fructose-bisphosphate aldolase A 

Protein Synonyms/Alias

 Muscle-type aldolase 

Gene Name

 Aldoa 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
13	PALTPEQKKELADIA	acetylation	[1]
14	ALTPEQKKELADIAH	acetylation	[1]
14	ALTPEQKKELADIAH	ubiquitination	[2]
28	HRIVAPGKGILAADE	acetylation	[1]
28	HRIVAPGKGILAADE	ubiquitination	[2]
42	ESTGSIAKRLQSIGT	acetylation	[1]
42	ESTGSIAKRLQSIGT	ubiquitination	[2]
108	KGGVVGIKVDKGVVP	acetylation	[1]
108	KGGVVGIKVDKGVVP	ubiquitination	[2]
111	VVGIKVDKGVVPLAG	acetylation	[1]
111	VVGIKVDKGVVPLAG	ubiquitination	[2]
140	ERCAQYKKDGADFAK	ubiquitination	[2]
147	KDGADFAKWRCVLKI	acetylation	[1]
147	KDGADFAKWRCVLKI	ubiquitination	[2]
153	AKWRCVLKIGEHTPS	acetylation	[1]
200	PDGDHDLKRCQYVTE	acetylation	[1]
230	YLEGTLLKPNMVTPG	acetylation	[1]
294	INKCPLLKPWALTFS	acetylation	[1]
312	ALQASALKAWGGKKE	acetylation	[1]
312	ALQASALKAWGGKKE	ubiquitination	[2]
317	ALKAWGGKKENLKAA	acetylation	[1]
322	GGKKENLKAAQEEYI	acetylation	[1]
322	GGKKENLKAAQEEYI	ubiquitination	[2]
330	AAQEEYIKRALANSL	acetylation	[1]
330	AAQEEYIKRALANSL	ubiquitination	[2]
342	NSLACQGKYTPSGQS	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity). 

Sequence Annotation

 ACT_SITE 188 188 Proton acceptor (By similarity).
 ACT_SITE 230 230 Schiff-base intermediate with
 BINDING 56 56 Substrate.
 BINDING 147 147 Substrate.
 MOD_RES 5 5 Phosphotyrosine (By similarity).
 MOD_RES 9 9 Phosphothreonine (By similarity).
 MOD_RES 13 13 N6-acetyllysine (By similarity).
 MOD_RES 36 36 Phosphoserine (By similarity).
 MOD_RES 37 37 Phosphothreonine (By similarity).
 MOD_RES 39 39 Phosphoserine (By similarity).
 MOD_RES 42 42 N6-acetyllysine (By similarity).
 MOD_RES 46 46 Phosphoserine (By similarity).
 MOD_RES 65 65 Phosphothreonine (By similarity).
 MOD_RES 108 108 N6-acetyllysine (By similarity).
 MOD_RES 111 111 N6-malonyllysine (By similarity).
 MOD_RES 204 204 Phosphotyrosine (By similarity).
 MOD_RES 223 223 Phosphotyrosine (By similarity).
 MOD_RES 235 235 Phosphothreonine (By similarity).
 MOD_RES 241 241 Phosphothreonine (By similarity).
 MOD_RES 312 312 N6-malonyllysine (By similarity).
 MOD_RES 330 330 N6-acetyllysine (By similarity).
 MOD_RES 354 354 Phosphoserine (By similarity).
 MOD_RES 356 356 Phosphoserine (By similarity).
 MOD_RES 364 364 Phosphotyrosine (By similarity).  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Glycolysis; Lyase; Phosphoprotein; Reference proteome; Schiff base. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 364 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:RGD.
 GO:0004332; F:fructose-bisphosphate aldolase activity; ISS:UniProtKB.
 GO:0006096; P:glycolysis; ISS:UniProtKB.
 GO:0051289; P:protein homotetramerization; ISS:UniProtKB.
 GO:0043627; P:response to estrogen stimulus; IEP:RGD.
 GO:0009408; P:response to heat; IEP:RGD.
 GO:0001666; P:response to hypoxia; IEP:RGD.
 GO:0032496; P:response to lipopolysaccharide; IEP:RGD.
 GO:0035094; P:response to nicotine; IEP:RGD. 

Interpro

 IPR000741; Aldolase_I.
 IPR013785; Aldolase_TIM. 

Pfam

 PF00274; Glycolytic 

SMART

  

PROSITE

 PS00158; ALDOLASE_CLASS_I 

PRINTS