UniProt Accession

 COPE_YEAST; P40509; D6VVK8 

Genbank Protein ID

 AF043582; Z37997; BK006942 

Genbank Nucleotide ID

 AAC14023.1; CAA86094.1; DAA08474.1 

Protein Name

 Coatomer subunit epsilon 

Protein Synonyms/Alias

 Epsilon-coat protein; Epsilon-COP 

Gene Name

 SEC28 

Gene Synonyms/Alias

 YIL076W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
37	DNTLLFYKAKTLLAL	ubiquitination	[1]
39	TLLFYKAKTLLALGQ	ubiquitination	[1]
276	DHEHAFIKHHQEIDA	acetylation	[2]
276	DHEHAFIKHHQEIDA	ubiquitination	[3]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

 The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). 

Sequence Annotation

  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Cytoplasmic vesicle; ER-Golgi transport; Golgi apparatus; Membrane; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 296 AA 

Protein Sequence

Gene Ontology

 GO:0030126; C:COPI vesicle coat; IMP:SGD.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; IMP:SGD.
 GO:0032511; P:late endosome to vacuole transport via multivesicular body sorting pathway; IMP:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0006890; P:retrograde vesicle-mediated transport, Golgi to ER; IEA:InterPro.
 GO:0006901; P:vesicle coating; IGI:SGD. 

Interpro

 IPR006822; Coatomer_esu. 

Pfam

 PF04733; Coatomer_E 

SMART

  

PROSITE

  

PRINTS