CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000409
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein CASC3 
Protein Synonyms/Alias
 Cancer susceptibility candidate gene 3 protein; Metastatic lymph node gene 51 protein; MLN 51; Protein barentsz; Btz 
Gene Name
 CASC3 
Gene Synonyms/Alias
 MLN51 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
128DAVNSSTKEEKGEEKubiquitination[1]
139GEEKPDTKSTVTGERubiquitination[2]
161STEPVENKVGKKGPKubiquitination[1, 2]
177LDDDEDRKNPAYIPRubiquitination[2]
185NPAYIPRKGLFFEHDubiquitination[2]
205QEEEVRPKGRQRKLWubiquitination[2]
254AHNPDDIKPRRIRKPubiquitination[2]
344GRSGETVKHEISYRSubiquitination[2, 3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Stimulates the ATPase and RNA- helicase activities of EIF4A3. Plays a role in the stress response by participating in cytoplasmic stress granules assembly and by favoring cell recovery following stress. Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons (By similarity). May play a role in mRNA transport (By similarity). Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Binds poly(G) and poly(U) RNA homopolymer. 
Sequence Annotation
 REGION 137 283 Necessary for RNA-binding, interaction
 REGION 137 283 Sufficient to form the EJC.
 REGION 377 703 Necessary for localization in cytoplasmic
 MOTIF 204 210 Nuclear localization signal 1
 MOTIF 254 262 Nuclear localization signal 2
 MOTIF 462 466 Nuclear export signal.
 MOD_RES 117 117 Phosphoserine.
 MOD_RES 148 148 Phosphoserine.
 MOD_RES 265 265 Phosphoserine.
 MOD_RES 363 363 Phosphoserine.
 MOD_RES 373 373 Phosphoserine.
 MOD_RES 477 477 Phosphoserine.  
Keyword
 3D-structure; ADP-ribosylation; Coiled coil; Complete proteome; Cytoplasm; mRNA processing; mRNA splicing; mRNA transport; Nonsense-mediated mRNA decay; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Stress response; Translation regulation; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 703 AA 
Protein Sequence
MADRRRQRAS QDTEDEESGA SGSDSGGSPL RGGGSCSGSA GGGGSGSLPS QRGGRTGALH 60
LRRVESGGAK SAEESECESE DGIEGDAVLS DYESAEDSEG EEGEYSEEEN SKVELKSEAN 120
DAVNSSTKEE KGEEKPDTKS TVTGERQSGD GQESTEPVEN KVGKKGPKHL DDDEDRKNPA 180
YIPRKGLFFE HDLRGQTQEE EVRPKGRQRK LWKDEGRWEH DKFREDEQAP KSRQELIALY 240
GYDIRSAHNP DDIKPRRIRK PRYGSPPQRD PNWNGERLNK SHRHQGLGGT LPPRTFINRN 300
AAGTGRMSAP RNYSRSGGFK EGRAGFRPVE AGGQHGGRSG ETVKHEISYR SRRLEQTSVR 360
DPSPEADAPV LGSPEKEEAA SEPPAAAPDA APPPPDRPIE KKSYSRARRT RTKVGDAVKL 420
AEEVPPPPEG LIPAPPVPET TPTPPTKTGT WEAPVDSSTS GLEQDVAQLN IAEQNWSPGQ 480
PSFLQPRELR GMPNHIHMGA GPPPQFNRME EMGVQGGRAK RYSSQRQRPV PEPPAPPVHI 540
SIMEGHYYDP LQFQGPIYTH GDSPAPLPPQ GMLVQPGMNL PHPGLHPHQT PAPLPNPGLY 600
PPPVSMSPGQ PPPQQLLAPT YFSAPGVMNF GNPSYPYAPG ALPPPPPPHL YPNTQAPSQV 660
YGGVTYYNPA QQQVQPKPSP PRRTPQPVTI KPPPPEVVSR GSS 703 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0035145; C:exon-exon junction complex; IDA:UniProtKB.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0030529; C:ribonucleoprotein complex; IEA:Compara.
 GO:0042802; F:identical protein binding; IDA:MGI.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0010467; P:gene expression; TAS:Reactome.
 GO:0008298; P:intracellular mRNA localization; IEA:Compara.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 
Interpro
 IPR018545; Btz_dom. 
Pfam
 PF09405; Btz 
SMART
 SM01044; Btz 
PROSITE
  
PRINTS