UniProt Accession

 LEF1_MOUSE; P27782 

Genbank Protein ID

 X58636; D16503 

Genbank Nucleotide ID

 CAA41493.1; BAA03954.1 

Protein Name

 Lymphoid enhancer-binding factor 1 

Protein Synonyms/Alias

 LEF-1 

Gene Name

 Lef1 

Gene Synonyms/Alias

 Lef-1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
25	TDEMIPFKDEGDPQK	sumoylation	[1]
267	AIVTPQVKQEHPHTD	sumoylation	[1]

Reference

 [1] PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies.
 Sachdev S, Bruhn L, Sieber H, Pichler A, Melchior F, Grosschedl R.
 Genes Dev. 2001 Dec 1;15(23):3088-103. [PMID: 11731474] 

Functional Description

 Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (By similarity). Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIASG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. 

Sequence Annotation

 DNA_BIND 297 365 HMG box.
 REGION 1 60 CTNNB1-binding.
 MOD_RES 130 130 Phosphoserine (By similarity).
 MOD_RES 153 153 Phosphothreonine; by NLK (By similarity).
 MOD_RES 164 164 Phosphoserine; by NLK (By similarity).
 CROSSLNK 25 25 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 267 267 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Activator; Alternative splicing; Complete proteome; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Ubl conjugation; Wnt signaling pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 397 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0032993; C:protein-DNA complex; IEA:Compara.
 GO:0005667; C:transcription factor complex; IPI:MGI.
 GO:0070016; F:armadillo repeat domain binding; IBA:RefGenome.
 GO:0008013; F:beta-catenin binding; IBA:RefGenome.
 GO:0003682; F:chromatin binding; IDA:MGI.
 GO:0043027; F:cysteine-type endopeptidase inhibitor activity involved in apoptotic process; IEA:Compara.
 GO:0008301; F:DNA binding, bending; IDA:UniProtKB.
 GO:0035326; F:enhancer binding; IBA:RefGenome.
 GO:0030284; F:estrogen receptor activity; IEA:Compara.
 GO:0030331; F:estrogen receptor binding; IBA:RefGenome.
 GO:0045295; F:gamma-catenin binding; IBA:RefGenome.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; IGI:MGI.
 GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; IEA:Compara.
 GO:0001228; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IEA:Compara.
 GO:0043565; F:sequence-specific DNA binding; IBA:RefGenome.
 GO:0060033; P:anatomical structure regression; IMP:MGI.
 GO:0060561; P:apoptotic process involved in morphogenesis; IMP:MGI.
 GO:0042100; P:B cell proliferation; IMP:MGI.
 GO:0030509; P:BMP signaling pathway; IDA:MGI.
 GO:0060070; P:canonical Wnt receptor signaling pathway; IBA:RefGenome.
 GO:0060326; P:cell chemotaxis; IEA:Compara.
 GO:0071353; P:cellular response to interleukin-4; IEA:Compara.
 GO:0060710; P:chorio-allantoic fusion; IGI:MGI.
 GO:0021542; P:dentate gyrus development; IMP:MGI.
 GO:0030326; P:embryonic limb morphogenesis; IGI:MGI.
 GO:0001837; P:epithelial to mesenchymal transition; IMP:BHF-UCL.
 GO:0048069; P:eye pigmentation; IBA:RefGenome.
 GO:0060325; P:face morphogenesis; IGI:MGI.
 GO:0021873; P:forebrain neuroblast division; IGI:MGI.
 GO:0021879; P:forebrain neuron differentiation; IBA:RefGenome.
 GO:0021861; P:forebrain radial glial cell differentiation; IGI:MGI.
 GO:0021943; P:formation of radial glial scaffolds; IGI:MGI.
 GO:0043966; P:histone H3 acetylation; IEA:Compara.
 GO:0043967; P:histone H4 acetylation; IEA:Compara.
 GO:0021854; P:hypothalamus development; IBA:RefGenome.
 GO:0006917; P:induction of apoptosis; IMP:MGI.
 GO:0030520; P:intracellular estrogen receptor signaling pathway; IBA:RefGenome.
 GO:0030879; P:mammary gland development; IMP:MGI.
 GO:0048747; P:muscle fiber development; IBA:RefGenome.
 GO:0071866; P:negative regulation of apoptotic process in bone marrow; IBA:RefGenome.
 GO:0090090; P:negative regulation of canonical Wnt receptor signaling pathway; IEA:Compara.
 GO:0022408; P:negative regulation of cell-cell adhesion; IEA:Compara.
 GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; IBA:RefGenome.
 GO:0043392; P:negative regulation of DNA binding; IEA:Compara.
 GO:0071899; P:negative regulation of estrogen receptor binding; IEA:Compara.
 GO:0032696; P:negative regulation of interleukin-13 production; IBA:RefGenome.
 GO:0032713; P:negative regulation of interleukin-4 production; IBA:RefGenome.
 GO:0032714; P:negative regulation of interleukin-5 production; IBA:RefGenome.
 GO:0045843; P:negative regulation of striated muscle tissue development; IDA:MGI.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0001755; P:neural crest cell migration; IBA:RefGenome.
 GO:0030223; P:neutrophil differentiation; IBA:RefGenome.
 GO:0071895; P:odontoblast differentiation; IBA:RefGenome.
 GO:0042475; P:odontogenesis of dentin-containing tooth; IMP:MGI.
 GO:0001649; P:osteoblast differentiation; IBA:RefGenome.
 GO:0060021; P:palate development; IMP:BHF-UCL.
 GO:0048341; P:paraxial mesoderm formation; IGI:MGI.
 GO:0001569; P:patterning of blood vessels; IMP:MGI.
 GO:0043923; P:positive regulation by host of viral transcription; IEA:Compara.
 GO:0090068; P:positive regulation of cell cycle process; IEA:Compara.
 GO:0030307; P:positive regulation of cell growth; IEA:Compara.
 GO:0030335; P:positive regulation of cell migration; IEA:Compara.
 GO:0071864; P:positive regulation of cell proliferation in bone marrow; IBA:RefGenome.
 GO:0022409; P:positive regulation of cell-cell adhesion; IEA:Compara.
 GO:0010718; P:positive regulation of epithelial to mesenchymal transition; IEA:Compara.
 GO:0030854; P:positive regulation of granulocyte differentiation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0022407; P:regulation of cell-cell adhesion; IMP:MGI.
 GO:0030111; P:regulation of Wnt receptor signaling pathway; IGI:MGI.
 GO:0050909; P:sensory perception of taste; IMP:MGI.
 GO:0001756; P:somitogenesis; IMP:MGI.
 GO:0002040; P:sprouting angiogenesis; IMP:MGI.
 GO:0033153; P:T cell receptor V(D)J recombination; IGI:MGI.
 GO:0045063; P:T-helper 1 cell differentiation; IDA:UniProtKB.
 GO:0043586; P:tongue development; IMP:MGI.
 GO:0061153; P:trachea gland development; IMP:MGI. 

Interpro

 IPR027397; Catenin_binding_dom.
 IPR013558; CTNNB1-bd_N.
 IPR009071; HMG_box_dom.
 IPR024940; TCF/LEF. 

Pfam

 PF08347; CTNNB1_binding
 PF00505; HMG_box 

SMART

 SM00398; HMG 

PROSITE

 PS50118; HMG_BOX_2 

PRINTS