CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000675
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kunitz-type protease inhibitor 2 
Protein Synonyms/Alias
 Hepatocyte growth factor activator inhibitor type 2; HAI-2; Placental bikunin 
Gene Name
 SPINT2 
Gene Synonyms/Alias
 HAI2; KOP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
87TKEECLKKCATVTENubiquitination[1]
242VWSSGDDKEQLVKNTubiquitination[1, 2, 3, 4, 5]
247DDKEQLVKNTYVL**ubiquitination[1, 2, 3, 4, 5, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
 Inhibitor of HGF activator. Also inhibits plasmin, plasma and tissue kallikrein, and factor XIa. 
Sequence Annotation
 DOMAIN 38 88 BPTI/Kunitz inhibitor 1.
 DOMAIN 133 183 BPTI/Kunitz inhibitor 2.
 CARBOHYD 57 57 N-linked (GlcNAc...).
 CARBOHYD 94 94 N-linked (GlcNAc...) (Potential).
 DISULFID 38 88 By similarity.
 DISULFID 47 71 By similarity.
 DISULFID 63 84 By similarity.
 DISULFID 133 183 By similarity.
 DISULFID 142 166 By similarity.
 DISULFID 158 179 By similarity.  
Keyword
 Alternative splicing; Complete proteome; Direct protein sequencing; Disease mutation; Disulfide bond; Glycoprotein; Membrane; Polymorphism; Protease inhibitor; Reference proteome; Repeat; Serine protease inhibitor; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 252 AA 
Protein Sequence
MAQLCGLRRS RAFLALLGSL LLSGVLAADR ERSIHDFCLV SKVVGRCRAS MPRWWYNVTD 60
GSCQLFVYGG CDGNSNNYLT KEECLKKCAT VTENATGDLA TSRNAADSSV PSAPRRQDSE 120
DHSSDMFNYE EYCTANAVTG PCRASFPRWY FDVERNSCNN FIYGGCRGNK NSYRSEEACM 180
LRCFRQQENP PLPLGSKVVV LAGLFVMVLI LFLGASMVYL IRVARRNQER ALRTVWSSGD 240
DKEQLVKNTY VL 252 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HGNC.
 GO:0005576; C:extracellular region; TAS:ProtInc.
 GO:0016021; C:integral to membrane; TAS:ProtInc.
 GO:0004867; F:serine-type endopeptidase inhibitor activity; TAS:ProtInc.
 GO:0006928; P:cellular component movement; TAS:ProtInc. 
Interpro
 IPR002223; Prot_inh_Kunz-m.
 IPR020901; Prtase_inh_Kunz-CS. 
Pfam
 PF00014; Kunitz_BPTI 
SMART
 SM00131; KU 
PROSITE
 PS00280; BPTI_KUNITZ_1
 PS50279; BPTI_KUNITZ_2 
PRINTS
 PR00759; BASICPTASE.