CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024368
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Agmatinase, mitochondrial 
Protein Synonyms/Alias
 Agmatine ureohydrolase; AUH 
Gene Name
 Agmat 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
199AHTNTTDKPREEKVYacetylation[1, 2, 3, 4]
204TDKPREEKVYHRTPFacetylation[2, 4]
223DEGLLDSKRVVQIGIacetylation[2, 3, 4, 5, 6, 7]
223DEGLLDSKRVVQIGIsuccinylation[7]
259LAEDCWMKSLVPLMAacetylation[3]
Reference
 [1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [6] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [7] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337
Functional Description
  
Sequence Annotation
 METAL 168 168 Manganese 1 (By similarity).
 METAL 193 193 Manganese 2 (By similarity).
 METAL 284 284 Manganese 2 (By similarity).  
Keyword
 Complete proteome; Hydrolase; Manganese; Metal-binding; Mitochondrion; Putrescine biosynthesis; Reference proteome; Spermidine biosynthesis; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 358 AA 
Protein Sequence
MLRLLRSSWA RGLGSGVATW RPSAGLFRPG CPGIRQASGA SDTPHHQSPS SESPVQPVGV 60
GVCSMMRLPL QSSPEGLDAA FIGVPLDTGT SNRPGARFGP CRIREESLML GAVNPSTGAL 120
PFQSLRVADL GNVNVNLYNL QDSCLLIREA YQNVLAAGCI PLTLGGDQTI TYPILQAVAK 180
EHGPVGLVHV GAHTNTTDKP REEKVYHRTP FRRSVDEGLL DSKRVVQIGI RGSSRTLDPY 240
RYSRSQGFRV VLAEDCWMKS LVPLMAEVRQ QMGGKPLYIS FAIDALDPAY APGTGTPEIA 300
GLTPSQALEI IRGCQGLNVV GCDLVEVSPP YDLSGNTALL AANLLFEMLC ALPKVTTV 358 
Gene Ontology
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0008783; F:agmatinase activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0033388; P:putrescine biosynthetic process from arginine; IEA:UniProtKB-UniPathway.
 GO:0008295; P:spermidine biosynthetic process; IEA:UniProtKB-KW. 
Interpro
 IPR005925; Agmatinase-rel.
 IPR006035; Ureohydrolase.
 IPR023696; Ureohydrolase_domain. 
Pfam
 PF00491; Arginase 
SMART
  
PROSITE
 PS01053; ARGINASE_1
 PS51409; ARGINASE_2 
PRINTS
 PR00116; ARGINASE.