CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013213
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 2,5-diketo-D-gluconic acid reductase A 
Protein Synonyms/Alias
 2,5-DKG reductase A; 2,5-DKGR A; 25DKGR-A; AKR5C 
Gene Name
 dkgA 
Gene Synonyms/Alias
 yqhE; b3012; JW5499 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
52IDTAAAYKNEEGVGKacetylation[1]
59KNEEGVGKALKNASVacetylation[1]
76EELFITTKLWNDDHKacetylation[1]
83KLWNDDHKRPREALLacetylation[1]
94EALLDSLKKLQLDYIacetylation[1]
128KGMIELQKEGLIKSIacetylation[1]
133LQKEGLIKSIGVCNFacetylation[1]
180HAWNATHKIQTESWSacetylation[1]
194SPLAQGGKGVFDQKVacetylation[1]
200GKGVFDQKVIRDLADacetylation[1]
208VIRDLADKYGKTPAQacetylation[1]
231SGLVVIPKSVTPSRIacetylation[1]
251VWDFRLDKDELGEIAacetylation[1]
259DELGEIAKLDQGKRLacetylation[1]
264IAKLDQGKRLGPDPDacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). It is also capable of stereoselective -keto ester reductions on ethyl acetoacetate and other 2-substituted derivatives. 
Sequence Annotation
 NP_BIND 187 241 NADP (By similarity).
 ACT_SITE 51 51 Proton donor (By similarity).
 BINDING 107 107 Substrate (By similarity).  
Keyword
 3D-structure; Ascorbate biosynthesis; Complete proteome; Cytoplasm; NADP; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 275 AA 
Protein Sequence
MANPTVIKLQ DGNVMPQLGL GVWQASNEEV ITAIQKALEV GYRSIDTAAA YKNEEGVGKA 60
LKNASVNREE LFITTKLWND DHKRPREALL DSLKKLQLDY IDLYLMHWPV PAIDHYVEAW 120
KGMIELQKEG LIKSIGVCNF QIHHLQRLID ETGVTPVINQ IELHPLMQQR QLHAWNATHK 180
IQTESWSPLA QGGKGVFDQK VIRDLADKYG KTPAQIVIRW HLDSGLVVIP KSVTPSRIAE 240
NFDVWDFRLD KDELGEIAKL DQGKRLGPDP DQFGG 275 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0050580; F:2,5-didehydrogluconate reductase activity; IEA:EC.
 GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IDA:EcoCyc.
 GO:0019853; P:L-ascorbic acid biosynthetic process; IEA:UniProtKB-KW.
 GO:0051596; P:methylglyoxal catabolic process; IMP:EcoCyc. 
Interpro
 IPR001395; Aldo/ket_red.
 IPR018170; Aldo/ket_reductase_CS.
 IPR020471; Aldo/keto_reductase_subgr.
 IPR023210; NADP_OxRdtase_dom. 
Pfam
 PF00248; Aldo_ket_red 
SMART
  
PROSITE
 PS00798; ALDOKETO_REDUCTASE_1
 PS00062; ALDOKETO_REDUCTASE_2
 PS00063; ALDOKETO_REDUCTASE_3 
PRINTS
 PR00069; ALDKETRDTASE.