CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-036996
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Translation initiation factor eIF-2B subunit delta 
Protein Synonyms/Alias
  
Gene Name
 EIF2B4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
163GETPSGVKRLPEYPQubiquitination[1]
197TRKDYGSKVSLFSHLubiquitination[1, 2, 3, 4]
303NAIKFLNKEITSVGSubiquitination[1, 2, 3, 4, 5, 6]
463LVCCETYKFCERVQTubiquitination[1]
487DPDDLQCKRGEHVALubiquitination[1, 5, 6, 7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 544 AA 
Protein Sequence
MPTQQPAAPS TRAPKPSRSL SGSLCALFSD ADSGSGMKAE LPPGPGAVGR EMTKEEKLQL 60
RKEKKQQKKK RKEEKGAEPE TGSAVSAAQC QVGPTRELPE SGIQLGTPRE KVPAGRSKAE 120
LRAERRAKQE AERALKQARK GEQGGPPPKA SPSTAGETPS GVKRLPEYPQ VDDLLLRRLV 180
KKPERQQVPT RKDYGSKVSL FSHLPQYSRQ NSLTQFMSIP SSVIHPAMVR LGLQYSQGLV 240
SGSNARCIAL LRALQQVIQD YTTPPNEELS RDLVNKLKPY MSFLTQCRPL SASMHNAIKF 300
LNKEITSVGS SKREEEAKSE LRAAIDRYVQ EKIVLAAQAI SRFAYQKISN GDVILVYGCS 360
SLVSRILQEA WTEGRRFRVV VVDSRPWLEG RHTLRSLVHA GVPASYLLIP AASYVLPEVS 420
KVLLGAHALL ANGSVMSRVG TAQLALVARA HNVPVLVCCE TYKFCERVQT DAFVSNELDD 480
PDDLQCKRGE HVALANWQNH ASLRLLNLVY DVTPPELVDL VITELGMIPC SSVPVVLRVK 540
SSDQ 544 
Gene Ontology
 GO:0044237; P:cellular metabolic process; IEA:InterPro. 
Interpro
 IPR000649; IF-2B-related. 
Pfam
 PF01008; IF-2B 
SMART
  
PROSITE
  
PRINTS