CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002456
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bifunctional purine biosynthetic protein ADE5,7 
Protein Synonyms/Alias
 Phosphoribosylamine--glycine ligase; Glycinamide ribonucleotide synthetase; GARS; Phosphoribosylglycinamide synthetase; Phosphoribosylformylglycinamidine cyclo-ligase; AIR synthase; AIRS; Phosphoribosyl-aminoimidazole synthetase 
Gene Name
 ADE5,7 
Gene Synonyms/Alias
 YGL234W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
19REHVLVTKLAQSPTVubiquitination[1]
28AQSPTVGKIYVAPGNubiquitination[1]
230KRIFDGDKGLNTGGMacetylation[2]
260TIDSQIVKPTIDGMRacetylation[2]
800CVIENGTKLY*****ubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
  
Sequence Annotation
 DOMAIN 114 330 ATP-grasp.
 NP_BIND 140 203 ATP (By similarity).
 REGION 1 450 GARS.
 REGION 451 802 AIRS.
 METAL 298 298 Manganese (By similarity).
 METAL 300 300 Manganese (By similarity).
 MOD_RES 455 455 Phosphoserine.
 MOD_RES 458 458 Phosphoserine.  
Keyword
 ATP-binding; Complete proteome; Direct protein sequencing; Ligase; Manganese; Metal-binding; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Purine biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 802 AA 
Protein Sequence
MLNILVLGNG AREHVLVTKL AQSPTVGKIY VAPGNGGTAT MDPSRVINWD ITPDVANFAR 60
LQSMAVEHKI NLVVPGPELP LVNGITSVFH SVGIPVFGPS VKAAQLEASK AFSKRFMSKH 120
NIPTASYDVF TNPEEAISFL QAHTDKAFVI KADGIAAGKG VIIPSSIDES VQAIKDIMVT 180
KQFGEEAGKQ VVIEQFLEGD EISLLTIVDG YSHFNLPVAQ DHKRIFDGDK GLNTGGMGAY 240
APAPVATPSL LKTIDSQIVK PTIDGMRRDG MPFVGVLFTG MILVKDSKTN QLVPEVLEYN 300
VRFGDPETQA VLSLLDDQTD LAQVFLAAAE HRLDSVNIGI DDTRSAVTVV VAAGGYPESY 360
AKGDKITLDT DKLPPHTQIF QAGTKYDSAT DSLLTNGGRV LSVTSTAQDL RTAVDTVYEA 420
VKCVHFQNSY YRKDIAYRAF QNSESSKVAI TYADSGVSVD NGNNLVQTIK EMVRSTRRPG 480
ADSDIGGFGG LFDLAQAGFR QNEDTLLVGA TDGVGTKLII AQETGIHNTV GIDLVAMNVN 540
DLVVQGAEPL FFLDYFATGA LDIQVASDFV SGVANGCIQS GCALVGGETS EMPGMYPPGH 600
YDTNGTAVGA VLRQDILPKI NEMAAGDVLL GLASSGVHSN GFSLVRKIIQ HVALPWDAPC 660
PWDESKTLGE GILEPTKIYV KQLLPSIRQR LLLGLAHITG GGLVENIPRA IPDHLQARVD 720
MSTWEVPRVF KWFGQAGNVP HDDILRTFNM GVGMVLIVKR ENVKAVCDSL TEEGEIIWEL 780
GSLQERPKDA PGCVIENGTK LY 802 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004637; F:phosphoribosylamine-glycine ligase activity; ISS:SGD.
 GO:0004641; F:phosphoribosylformylglycinamidine cyclo-ligase activity; ISS:SGD.
 GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0009113; P:purine nucleobase biosynthetic process; IEA:InterPro.
 GO:0006144; P:purine nucleobase metabolic process; TAS:SGD. 
Interpro
 IPR010918; AIR_synth_C_dom.
 IPR000728; AIR_synth_N_dom.
 IPR011761; ATP-grasp.
 IPR013815; ATP_grasp_subdomain_1.
 IPR013816; ATP_grasp_subdomain_2.
 IPR016185; PreATP-grasp_dom.
 IPR020561; PRibGlycinamid_synth_ATP-grasp.
 IPR000115; PRibGlycinamide_synth.
 IPR020560; PRibGlycinamide_synth_C-dom.
 IPR020559; PRibGlycinamide_synth_CS.
 IPR020562; PRibGlycinamide_synth_N.
 IPR004733; PurM_cligase.
 IPR016188; PurM_N-like.
 IPR011054; Rudment_hybrid_motif. 
Pfam
 PF00586; AIRS
 PF02769; AIRS_C
 PF01071; GARS_A
 PF02843; GARS_C
 PF02844; GARS_N 
SMART
  
PROSITE
 PS50975; ATP_GRASP
 PS00184; GARS 
PRINTS