CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023910
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CD2-associated protein 
Protein Synonyms/Alias
 Adapter protein CMS; Cas ligand with multiple SH3 domains 
Gene Name
 CD2AP 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
102IQPHPQTKNIKKKTKubiquitination[1]
436EVSSISSKFETEPVSubiquitination[1]
520HSPEKILKLPKEEDSubiquitination[2, 3]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. May play a role in receptor clustering and cytoskeletal polarity in the junction between T- cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis. 
Sequence Annotation
 DOMAIN 1 59 SH3 1; truncated.
 DOMAIN 108 167 SH3 2.
 DOMAIN 269 330 SH3 3.
 REGION 1 175 Interaction with ANLN and localization to
 MOTIF 336 352 SH3-binding (Potential).
 MOTIF 378 397 SH3-binding (Potential).
 MOTIF 410 422 SH3-binding (Potential).
 MOD_RES 224 224 Phosphoserine.
 MOD_RES 458 458 Phosphoserine.
 MOD_RES 510 510 Phosphoserine.
 MOD_RES 514 514 Phosphoserine.  
Keyword
 3D-structure; Cell cycle; Cell division; Cell projection; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Mitosis; Phosphoprotein; Polymorphism; Reference proteome; Repeat; SH3 domain; SH3-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 639 AA 
Protein Sequence
MVDYIVEYDY DAVHDDELTI RVGEIIRNVK KLQEEGWLEG ELNGRRGMFP DNFVKEIKRE 60
TEFKDDSLPI KRERHGNVAS LVQRISTYGL PAGGIQPHPQ TKNIKKKTKK RQCKVLFEYI 120
PQNEDELELK VGDIIDINEE VEEGWWSGTL NNKLGLFPSN FVKELEVTDD GETHEAQDDS 180
ETVLAGPTSP IPSLGNVSET ASGSVTQPKK IRGIGFGDIF KEGSVKLRTR TSSSETEEKK 240
PEKPLILQSL GPKTQSVEIT KTDTEGKIKA KEYCRTLFAY EGTNEDELTF KEGEIIHLIS 300
KETGEAGWWR GELNGKEGVF PDNFAVQINE LDKDFPKPKK PPPPAKAPAP KPELIAAEKK 360
YFSLKPEEKD EKSTLEQKPS KPAAPQVPPK KPTPPTKASN LLRSSGTVYP KRPEKPVPPP 420
PPIAKINGEV SSISSKFETE PVSKLKLDSE QLPLRPKSVD FDSLTVRTSK ETDVVNFDDI 480
ASSENLLHLT ANRPKMPGRR LPGRFNGGHS PTHSPEKILK LPKEEDSANL KPSELKKDTC 540
YSPKPSVYLS TPSSASKANT TAFLTPLEIK AKVETDDVKK NSLDELRAQI IELLCIVEAL 600
KKDHGKELEK LRKDLEEEKT MRSNLEMEIE KLKKAVLSS 639 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0031941; C:filamentous actin; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0001726; C:ruffle; IDA:UniProtKB.
 GO:0005200; F:structural constituent of cytoskeleton; TAS:ProtInc.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0006461; P:protein complex assembly; TAS:ProtInc.
 GO:0007165; P:signal transduction; NAS:ProtInc.
 GO:0006930; P:substrate-dependent cell migration, cell extension; TAS:ProtInc. 
Interpro
 IPR011511; SH3_2.
 IPR001452; SH3_domain. 
Pfam
 PF00018; SH3_1
 PF07653; SH3_2 
SMART
 SM00326; SH3 
PROSITE
 PS50002; SH3 
PRINTS
 PR00452; SH3DOMAIN.