CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-039833
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Dynactin subunit 2 
Protein Synonyms/Alias
  
Gene Name
 DCTN2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
42KDKRVGTKGLDFSDRubiquitination[1]
117LTPVLLAKQLAALKQubiquitination[1, 2, 3]
123AKQLAALKQQLVASHubiquitination[2]
152DPDGALAKRLLLQLEubiquitination[1, 3, 4, 5, 6]
266RLQSVLGKVNEIAKHubiquitination[1, 3]
274VNEIAKHKASVEDADubiquitination[4]
285EDADTQSKVHQLYETacetylation[7]
285EDADTQSKVHQLYETubiquitination[4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 378 AA 
Protein Sequence
MYAQGTEDSR TLEELTSTSV EHIIVNPNAA YDKFKDKRVG TKGLDFSDRI GKTKRTGYES 60
GEYEMLGEGL GVKETPQQKY QRLLHEVQEL TTEVEKIKTT VKESATEEKL TPVLLAKQLA 120
ALKQQLVASH LEKLLGPDAA INLTDPDGAL AKRLLLQLEA TKNSKGGSGG KTTGTPPDSS 180
LVTYELHSRP EQDKFSQAAK VAELEKRLTE LETAVRCDQD AQNPLSAGLQ GACLMETVEL 240
LQAKVSALDL AVLDQVEARL QSVLGKVNEI AKHKASVEDA DTQSKVHQLY ETIQRWSPIA 300
STLPELVQRL VTIKQLHEQA MQFGQLLTHL DTTQQMIANS LKDNTTLLTQ VQTTMRENLA 360
TVEGNFASID ERMKKLGK 378 
Gene Ontology
 GO:0005813; C:centrosome; IDA:HPA.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005869; C:dynactin complex; IEA:InterPro.
 GO:0007017; P:microtubule-based process; IEA:InterPro. 
Interpro
 IPR006996; Dynamitin_2su. 
Pfam
  
SMART
  
PROSITE
  
PRINTS