CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000500
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphonoacetaldehyde hydrolase 
Protein Synonyms/Alias
 Phosphonatase; Phosphonoacetaldehyde phosphonohydrolase 
Gene Name
 phnX 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Bacillus cereus 
NCBI Taxa ID
 1396 
Lysine Modification
Position
Peptide
Type
References
50RKPMGLLKIDHVRALacetylation[1]
118SLRERGIKIGSTTGYacetylation[1]
143EAALQGYKPDFLVTPacetylation[1]
189GDTVSDMKEGRNAGMacetylation[1]
Reference
 [1] Kinetic evidence for a substrate-induced fit in phosphonoacetaldehyde hydrolase catalysis.
 Zhang G, Mazurkie AS, Dunaway-Mariano D, Allen KN.
 Biochemistry. 2002 Nov 12;41(45):13370-7. [PMID: 12416981] 
Functional Description
 Involved in phosphonate degradation. 
Sequence Annotation
 ACT_SITE 9 9 Nucleophile.
 ACT_SITE 50 50 Schiff-base intermediate with substrate.
 METAL 9 9 Magnesium.
 METAL 11 11 Magnesium; via carbonyl oxygen.
 METAL 183 183 Magnesium.  
Keyword
 3D-structure; Direct protein sequencing; Hydrolase; Magnesium; Metal-binding; Schiff base. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 264 AA 
Protein Sequence
MKIEAVIFDW AGTTVDYGCF APLEVFMEIF HKRGVAITAE EARKPMGLLK IDHVRALTEM 60
PRIASEWNRV FRQLPTEADI QEMYEEFEEI LFAILPRYAS PINGVKEVIA SLRERGIKIG 120
STTGYTREMM DIVAKEAALQ GYKPDFLVTP DDVPAGRPYP WMCYKNAMEL GVYPMNHMIK 180
VGDTVSDMKE GRNAGMWTVG VILGSSELGL TEEEVENMDS VELREKIEVV RNRFVENGAH 240
FTIETMQELE SVMEHIEKQE LIIS 264 
Gene Ontology
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0008967; F:phosphoglycolate phosphatase activity; IEA:InterPro.
 GO:0050194; F:phosphonoacetaldehyde hydrolase activity; IEA:HAMAP.
 GO:0019700; P:organic phosphonate catabolic process; IEA:InterPro. 
Interpro
 IPR023214; HAD-like_dom.
 IPR006439; HAD-SF_hydro_IA_v1.
 IPR006402; HAD-SF_hydro_IA_v3.
 IPR023198; PGP_dom2.
 IPR006323; Phosphonoacetald_hydro. 
Pfam
 PF13419; HAD_2 
SMART
  
PROSITE
  
PRINTS