CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020702
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Superkiller viralicidic activity 2-like 2 
Protein Synonyms/Alias
 ATP-dependent helicase SKIV2L2 
Gene Name
 Skiv2l2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
41GPPGSADKAGKRLDTacetylation[1]
76TDEPIFGKKPRIEDSacetylation[1, 2, 3, 4]
374KGPSNVFKIVKMIMEacetylation[2]
435CLSDEDKKLPQVEHVubiquitination[5]
759DNRQSVLKSIQEVQRubiquitination[5]
794GLKKVIQKVEAFEHRubiquitination[5]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 May be involved in pre-mRNA splicing. Associated with the RNA exosome complex and involved in the 3'processing of the 7S pre-RNA to the mature 5.8S rRNA (By similarity). 
Sequence Annotation
 DOMAIN 146 302 Helicase ATP-binding.
 DOMAIN 403 575 Helicase C-terminal.
 NP_BIND 159 166 ATP (Potential).
 MOTIF 250 253 DEIH box.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 49 49 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; ATP-binding; Complete proteome; Helicase; Hydrolase; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Reference proteome; rRNA processing; Spliceosome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1040 AA 
Protein Sequence
MADAFGDELF SVFEDDSTSA AGAKKDKEKE KWKGPPGSAD KAGKRLDTKL QSESASGGKN 60
KRDLDVEGTD EPIFGKKPRI EDSINEDLSL ADLMPRVKVQ SVETVEGCTH EVALPADEDY 120
IPLKPRVGKA AKEYPFILDA FQREAIQCVD NNQSVLVSAH TSAGKTVCAE YAIALALREK 180
QRVIFTSPIK ALSNQKYREM YEEFQDVGLM TGDVTINPTA SCLVMTTEIL RSMLYRGSEV 240
MREVAWVIFD EIHYMRDSER GVVWEETIIL LPDNVHYVFL SATIPNARQF AEWICHLHKQ 300
PCHVIYTDYR PTPLQHYIFP AGGDGLHLVV DENGDFREDN FNTAMQVLRD AGDLAKGDQK 360
GRKGGTKGPS NVFKIVKMIM ERNFQPVIIF SFSKKDCEAY ALQMTKLDFN TDEEKKMVEE 420
VFNNAIDCLS DEDKKLPQVE HVLPLLKRGI GIHHGGLLPI LKETIEILFS EGLIKALFAT 480
ETFAMGINMP ARTVLFTNAR KYDGKDFRWI SSGEYIQMSG RAGRRGMDDR GIVILMVDEK 540
MSPTIGKQLL KGSADPLNSA FHLTYNMVLN LLRVEEINPE YMLEKSFYQF QHYRAIPGVV 600
EKVKNSEEQY NKIVIPNEEN VVIYYKIRQQ LAKLGKEIEE YIHKPKYCLP FLQPGRLVKV 660
KNEGDDFGWG VVVNFSKKSN VKPNSGELDP LYVVEVLLRC SKESLKNSAT EAAKPAKPDE 720
KGEMQVVPVL VHLLSAISTV RLYIPKDLRP VDNRQSVLKS IQEVQRRFPD GVPLLDPIDD 780
MGIQDQGLKK VIQKVEAFEH RMYSHPLHND PNLETVYTLC ERKAQIALDI KSAKRELKKA 840
RTVLQMDELK CRKRVLRRLG FATSSDVIEM KGRVACEISS ADELLLTEMM FNGLFNDLSS 900
EQATALLSCF VFQENSSEMP KLTEQLAGPL RQMQECAKRI AKVSAEAKLE IDEETYLSSF 960
KPHLMDVVYT WATGATFAHI CKMTDVFEGS IIRCMRRLEE LLRQMCQAAK AIGNTELENK 1020
FAEGITKIKR DIVFAASLYL 1040 
Gene Ontology
 GO:0071013; C:catalytic step 2 spliceosome; IEA:Compara.
 GO:0000178; C:exosome (RNase complex); IEA:Compara.
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0000460; P:maturation of 5.8S rRNA; IEA:Compara.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR012961; DSH_C.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR016438; RNA_helicase_ATP-dep_SK12/DOB1.
 IPR025696; rRNA_proc-arch_dom. 
Pfam
 PF00270; DEAD
 PF08148; DSHCT
 PF00271; Helicase_C
 PF13234; rRNA_proc-arch 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS