UniProt Accession

 PTGA_ECOLI; P69783; P08837; Q47703 

Genbank Protein ID

 J02796; M21994; M93578; M93579; M93580; M93581; M93582; M93584; M93587; M93594; M93595; M93596; M93597; M93598; U53700; U00096; AP009048 

Genbank Nucleotide ID

 AAA24442.1; AAA24386.1; AAA23602.1; AAA23605.1; AAA23603.1; AAA23606.1; AAA23604.1; AAA23610.1; AAA23612.1; AAA23607.1; AAA23608.1; AAA23611.1; AAA23609.1; AAA23613.1; AAC44167.1; AAC75470.1; BAA16291.1 

Protein Name

 Glucose-specific phosphotransferase enzyme IIA component 

Protein Synonyms/Alias

 EIIA-Glc; EIII-Glc; PTS system glucose-specific EIIA component 

Gene Name

 crr 

Gene Synonyms/Alias

 gsr; iex; tgs; treD; b2417; JW2410 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
6	**MGLFDKLKSLVSD	acetylation	[1]
8	MGLFDKLKSLVSDDK	acetylation	[1]
15	KSLVSDDKKDTGTIE	acetylation	[1]
54	VGDGIAIKPTGNKMV	acetylation	[1, 2]
59	AIKPTGNKMVAPVDG	acetylation	[1, 2]
70	PVDGTIGKIFETNHA	acetylation	[3]
115	AEEGQRVKVGDTVIE	acetylation	[1]
131	DLPLLEEKAKSTLTP	acetylation	[1]
133	PLLEEKAKSTLTPVV	acetylation	[1]
148	ISNMDEIKELIKLSG	acetylation	[1, 2, 4]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [3] Topography of the surface of the Escherichia coli phosphotransferase system protein enzyme IIAglc that interacts with lactose permease.
 Sondej M, Seok YJ, Badawi P, Koo BM, Nam TW, Peterkofsky A.
 Biochemistry. 2000 Mar 21;39(11):2931-9. [PMID: 10715113]
 [4] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508] 

Functional Description

 The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport. 

Sequence Annotation

 DOMAIN 39 143 PTS EIIA type-1.
 ACT_SITE 91 91 Tele-phosphohistidine intermediate.
 METAL 76 76 Zinc; shared with glycerol kinase.
 METAL 91 91 Zinc; shared with glycerol kinase.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Metal-binding; Phosphotransferase system; Reference proteome; Sugar transport; Transferase; Transport; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 169 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005351; F:sugar:hydrogen symporter activity; IEA:InterPro.
 GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW. 

Interpro

 IPR011055; Dup_hybrid_motif.
 IPR001127; PTS_EIIA_1_perm. 

Pfam

 PF00358; PTS_EIIA_1 

SMART

  

PROSITE

 PS51093; PTS_EIIA_TYPE_1
 PS00371; PTS_EIIA_TYPE_1_HIS 

PRINTS