UniProt Accession

 TPIS_RAT; P48500; A0JN18; Q6P793 

Genbank Protein ID

 L36250; BC061781; BC126087 

Genbank Nucleotide ID

 AAA42278.1; AAH61781.1; AAI26088.1 

Protein Name

 Triosephosphate isomerase 

Protein Synonyms/Alias

 TIM; Triose-phosphate isomerase 

Gene Name

 Tpi1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
131	VIACIGEKLDEREAG	acetylation	[1]
142	REAGITEKVVFEQTK	acetylation	[1]
142	REAGITEKVVFEQTK	ubiquitination	[2]
149	KVVFEQTKAIADNVK	acetylation	[1]
149	KVVFEQTKAIADNVK	ubiquitination	[2]
156	KAIADNVKDWCKVVL	acetylation	[1]
175	VWAIGTGKTATPQQA	ubiquitination	[2]
238	LVGGASLKPEFVDII	acetylation	[1]
248	FVDIINAKQ******	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

  

Sequence Annotation

 ACT_SITE 96 96 Electrophile (By similarity).
 ACT_SITE 166 166 Proton acceptor (By similarity).
 BINDING 12 12 Substrate (By similarity).
 BINDING 14 14 Substrate (By similarity).
 MOD_RES 14 14 N6-acetyllysine (By similarity).
 MOD_RES 68 68 Nitrated tyrosine (By similarity).
 MOD_RES 80 80 Phosphoserine (By similarity).
 MOD_RES 194 194 N6-acetyllysine (By similarity).
 MOD_RES 209 209 Nitrated tyrosine (By similarity).
 MOD_RES 238 238 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Gluconeogenesis; Glycolysis; Isomerase; Nitration; Pentose shunt; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 249 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:RGD.
 GO:0005634; C:nucleus; IDA:RGD.
 GO:0004807; F:triose-phosphate isomerase activity; IDA:RGD.
 GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniPathway.
 GO:0006096; P:glycolysis; TAS:RGD.
 GO:0006098; P:pentose-phosphate shunt; IEA:UniProtKB-KW. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR022896; TrioseP_Isoase_bac/euk.
 IPR000652; Triosephosphate_isomerase.
 IPR020861; Triosephosphate_isomerase_AS. 

Pfam

 PF00121; TIM 

SMART

  

PROSITE

 PS00171; TIM_1
 PS51440; TIM_2 

PRINTS