UniProt Accession

 THIL_YEAST; P41338; D6W3Y5 

Genbank Protein ID

 L20428; U36624; BK006949 

Genbank Nucleotide ID

 AAA62378.1; AAB68159.1; DAA11401.1 

Protein Name

 Acetyl-CoA acetyltransferase 

Protein Synonyms/Alias

 Acetoacetyl-CoA thiolase; Ergosterol biosynthesis protein 10 

Gene Name

 ERG10 

Gene Synonyms/Alias

 YPL028W; LPB3 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
25	FQGSLSSKTAVELGA	ubiquitination	[1]
96	KVCASAMKAIILGAQ	acetylation	[2]
136	PAARAGAKFGQTVLV	acetylation	[2]
197	QKSQKEGKFDNEIVP	acetylation	[2]
197	QKSQKEGKFDNEIVP	ubiquitination	[1]
208	EIVPVTIKGFRGKPD	acetylation	[2]
220	KPDTQVTKDEEPARL	acetylation	[2]
231	PARLHVEKLRSARTV	acetylation	[2]
241	SARTVFQKENGTVTA	acetylation	[2]
275	VLKEKNLKPLAIIKG	acetylation	[2]
337	LVNTKILKLDPSKVN	acetylation	[2]
342	ILKLDPSKVNVYGGA	ubiquitination	[1]
397	ASSIVIEKI******	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Catalyzes the formation of acetoacetyl-CoA in the biosynthesis of mevalonate, an intermediate required for the biosynthesis of sterols and nonsterol isoprenoids. 

Sequence Annotation

 ACT_SITE 91 91 Acyl-thioester intermediate (By
 ACT_SITE 354 354 Proton acceptor (By similarity).
 ACT_SITE 384 384 Proton acceptor (By similarity).
 METAL 186 186 Potassium (By similarity).
 METAL 248 248 Potassium; via carbonyl oxygen (By
 METAL 249 249 Potassium; via carbonyl oxygen (By
 METAL 251 251 Potassium; via carbonyl oxygen (By
 METAL 350 350 Potassium; via carbonyl oxygen (By
 BINDING 186 186 Coenzyme A (By similarity).
 BINDING 231 231 Coenzyme A (By similarity).
 BINDING 252 252 Coenzyme A (By similarity).
 MOD_RES 2 2 N-acetylserine.  

Keyword

 Acetylation; Acyltransferase; Complete proteome; Cytoplasm; Direct protein sequencing; Metal-binding; Potassium; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 398 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0003985; F:acetyl-CoA C-acetyltransferase activity; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006696; P:ergosterol biosynthetic process; IMP:SGD. 

Interpro

 IPR002155; Thiolase.
 IPR016039; Thiolase-like.
 IPR016038; Thiolase-like_subgr.
 IPR020615; Thiolase_acyl_enz_int_AS.
 IPR020610; Thiolase_AS.
 IPR020617; Thiolase_C.
 IPR020613; Thiolase_CS.
 IPR020616; Thiolase_N. 

Pfam

 PF02803; Thiolase_C
 PF00108; Thiolase_N 

SMART

  

PROSITE

 PS00098; THIOLASE_1
 PS00737; THIOLASE_2
 PS00099; THIOLASE_3 

PRINTS