UniProt Accession

 TRIP4_HUMAN; Q15650; B2RAS0; Q96ED7; Q9UKH0 

Genbank Protein ID

 AF168418; AK314319; CH471082; BC012448; L40371 

Genbank Nucleotide ID

 AAF01278.1; BAG36967.1; EAW77685.1; AAH12448.1; AAC41738.1 

Protein Name

 Activating signal cointegrator 1 

Protein Synonyms/Alias

 ASC-1; Thyroid receptor-interacting protein 4; TR-interacting protein 4; TRIP-4 

Gene Name

 TRIP4 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
135	KTPFDLAKAQENSNS	ubiquitination	[1]
245	GKVDISTKDLLPHQE	ubiquitination	[1, 2, 3]
300	DSNQWLSKLERETLQ	ubiquitination	[1]
334	TIDFAGRKILEEENS	ubiquitination	[4]
367	TLNQPLTKLDRSSEE	ubiquitination	[1, 4, 5]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Transcription coactivator of nuclear receptors which functions in conjunction with CBP-p300 and SRC-1 and may play an important role in establishing distinct coactivator complexes under different cellular conditions. Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF- kappa-B. Plays a role in androgen receptor transactivation and in testicular function (By similarity). 

Sequence Annotation

 ZN_FING 171 187 C4-type.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 289 289 Phosphotyrosine (By similarity).  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Cytoskeleton; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 581 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0016922; F:ligand-dependent nuclear receptor binding; IDA:UniProtKB.
 GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; NAS:UniProtKB.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc. 

Interpro

 IPR007374; ASCH_domain.
 IPR015947; PUA-like_domain.
 IPR009349; Znf_C2HC5. 

Pfam

 PF04266; ASCH
 PF06221; zf-C2HC5 

SMART

 SM01022; ASCH 

PROSITE

  

PRINTS