UniProt Accession

 COF1_RAT; P45592 

Genbank Protein ID

 X62908; BC059143; BC086533 

Genbank Nucleotide ID

 CAA44694.1; AAH59143.1; AAH86533.1 

Protein Name

 Cofilin-1 

Protein Synonyms/Alias

 Cofilin, non-muscle isoform 

Gene Name

 Cfl1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
30	SSTPEEVKKRKKAVL	acetylation	[1]
44	LFCLSEDKKNIILEE	acetylation	[1]
45	FCLSEDKKNIILEEG	ubiquitination	[2]
92	YDATYETKESKKEDL	ubiquitination	[2]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization (By similarity). 

Sequence Annotation

 DOMAIN 4 153 ADF-H.
 MOTIF 30 34 Nuclear localization signal (Potential).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 3 3 Phosphoserine.
 MOD_RES 8 8 Phosphoserine (By similarity).
 MOD_RES 13 13 N6-acetyllysine (By similarity).
 MOD_RES 25 25 Phosphothreonine (By similarity).
 MOD_RES 68 68 Phosphotyrosine (By similarity).
 MOD_RES 73 73 N6-acetyllysine (By similarity).
 MOD_RES 140 140 Phosphotyrosine (By similarity).
 MOD_RES 144 144 N6-acetyllysine (By similarity).
 MOD_RES 156 156 Phosphoserine (By similarity).  

Keyword

 Acetylation; Actin-binding; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Membrane; Nucleus; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 166 AA 

Protein Sequence

Gene Ontology

 GO:0030864; C:cortical actin cytoskeleton; IEA:Compara.
 GO:0005737; C:cytoplasm; IDA:RGD.
 GO:0030027; C:lamellipodium; IDA:RGD.
 GO:0031258; C:lamellipodium membrane; IEA:UniProtKB-SubCell.
 GO:0016363; C:nuclear matrix; IEA:UniProtKB-SubCell.
 GO:0032587; C:ruffle membrane; IEA:UniProtKB-SubCell.
 GO:0003779; F:actin binding; IMP:RGD.
 GO:0030042; P:actin filament depolymerization; IEA:InterPro.
 GO:0030030; P:cell projection organization; IDA:MGI.
 GO:0000910; P:cytokinesis; IEA:Compara.
 GO:0007010; P:cytoskeleton organization; ISS:UniProtKB.
 GO:0030010; P:establishment of cell polarity; IEA:Compara.
 GO:0045792; P:negative regulation of cell size; IDA:MGI.
 GO:0001755; P:neural crest cell migration; IEA:Compara.
 GO:0001842; P:neural fold formation; IEA:Compara.
 GO:0030836; P:positive regulation of actin filament depolymerization; IEA:Compara.
 GO:0006606; P:protein import into nucleus; IMP:RGD.
 GO:0006468; P:protein phosphorylation; IEA:Compara.
 GO:0022604; P:regulation of cell morphogenesis; ISS:UniProtKB.
 GO:0043200; P:response to amino acid stimulus; IEA:Compara. 

Interpro

 IPR002108; Actin-bd_cofilin/tropomyosin.
 IPR017904; ADF/Cofilin/Destrin.
 IPR027234; Cofilin_1/2. 

Pfam

 PF00241; Cofilin_ADF 

SMART

 SM00102; ADF 

PROSITE

 PS51263; ADF_H 

PRINTS

 PR00006; COFILIN.