CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011594
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Galectin-3-binding protein 
Protein Synonyms/Alias
 Basement membrane autoantigen p105; Lectin galactoside-binding soluble 3-binding protein; Mac-2-binding protein; MAC2BP; Mac-2 BP; Tumor-associated antigen 90K 
Gene Name
 LGALS3BP 
Gene Synonyms/Alias
 M2BP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
323PSELALLKAVDTWSWubiquitination[1, 2]
345EVEGLVEKIRFPMMLubiquitination[3, 4]
376HEALFQKKTLQALEFubiquitination[4]
395FQLLARYKGLNLTEDubiquitination[2, 4]
427DSSWSARKSQLVYQSubiquitination[4]
441SRRGPLVKYSSDYFQubiquitination[2, 4, 5, 6]
474PSFLFQDKRVSWSLVubiquitination[5, 6]
Reference
 [1] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells. 
Sequence Annotation
 DOMAIN 24 124 SRCR.
 DOMAIN 153 221 BTB.
 DOMAIN 260 360 BACK.
 CARBOHYD 69 69 N-linked (GlcNAc...).
 CARBOHYD 125 125 N-linked (GlcNAc...).
 CARBOHYD 192 192 N-linked (GlcNAc...).
 CARBOHYD 362 362 N-linked (GlcNAc...).
 CARBOHYD 398 398 N-linked (GlcNAc...).
 CARBOHYD 551 551 N-linked (GlcNAc...).
 CARBOHYD 580 580 N-linked (GlcNAc...).
 DISULFID 49 113
 DISULFID 62 123
 DISULFID 93 103  
Keyword
 3D-structure; Cell adhesion; Complete proteome; Direct protein sequencing; Disulfide bond; Extracellular matrix; Glycoprotein; Reference proteome; Secreted; Signal. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 585 AA 
Protein Sequence
MTPPRLFWVW LLVAGTQGVN DGDMRLADGG ATNQGRVEIF YRGQWGTVCD NLWDLTDASV 60
VCRALGFENA TQALGRAAFG QGSGPIMLDE VQCTGTEASL ADCKSLGWLK SNCRHERDAG 120
VVCTNETRST HTLDLSRELS EALGQIFDSQ RGCDLSISVN VQGEDALGFC GHTVILTANL 180
EAQALWKEPG SNVTMSVDAE CVPMVRDLLR YFYSRRIDIT LSSVKCFHKL ASAYGARQLQ 240
GYCASLFAIL LPQDPSFQMP LDLYAYAVAT GDALLEKLCL QFLAWNFEAL TQAEAWPSVP 300
TDLLQLLLPR SDLAVPSELA LLKAVDTWSW GERASHEEVE GLVEKIRFPM MLPEELFELQ 360
FNLSLYWSHE ALFQKKTLQA LEFHTVPFQL LARYKGLNLT EDTYKPRIYT SPTWSAFVTD 420
SSWSARKSQL VYQSRRGPLV KYSSDYFQAP SDYRYYPYQS FQTPQHPSFL FQDKRVSWSL 480
VYLPTIQSCW NYGFSCSSDE LPVLGLTKSG GSDRTIAYEN KALMLCEGLF VADVTDFEGW 540
KAAIPSALDT NSSKSTSSFP CPAGHFNGFR TVIRPFYLTN SSGVD 585 
Gene Ontology
 GO:0005615; C:extracellular space; TAS:ProtInc.
 GO:0070062; C:extracellular vesicular exosome; IDA:UniProtKB.
 GO:0016020; C:membrane; IEA:InterPro.
 GO:0005578; C:proteinaceous extracellular matrix; IEA:UniProtKB-SubCell.
 GO:0005044; F:scavenger receptor activity; TAS:ProtInc.
 GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
 GO:0006968; P:cellular defense response; TAS:ProtInc.
 GO:0007165; P:signal transduction; TAS:ProtInc. 
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR001190; Srcr_rcpt.
 IPR017448; Srcr_rcpt-rel. 
Pfam
 PF07707; BACK
 PF00530; SRCR 
SMART
 SM00875; BACK
 SM00202; SR 
PROSITE
 PS50097; BTB
 PS00420; SRCR_1
 PS50287; SRCR_2 
PRINTS
 PR00258; SPERACTRCPTR.