CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018075
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 GPI ethanolamine phosphate transferase 3 
Protein Synonyms/Alias
 Phosphatidylinositol-glycan biosynthesis class O protein; PIG-O 
Gene Name
 PIGO 
Gene Synonyms/Alias
 UNQ632/PRO1249 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
103VSLPFLGKLSSLQRIubiquitination[1, 2, 3, 4, 5]
136TTTMQRLKALTTGSLubiquitination[3, 6]
251HHPEMAKKLSQMDQVubiquitination[2, 3]
391ATQDLQAKELHQLQNubiquitination[3, 6]
402QLQNLFSKASADYQWubiquitination[6]
695LRRYGNLKSPEPPMLubiquitination[2, 6]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the GPI third mannose which links the GPI-anchor to the C-terminus of the proteins by an amide bond (By similarity). 
Sequence Annotation
 CARBOHYD 268 268 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Complete proteome; Disease mutation; Endoplasmic reticulum; Glycoprotein; GPI-anchor biosynthesis; Membrane; Mental retardation; Polymorphism; Reference proteome; Transferase; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1089 AA 
Protein Sequence
MQKASVLLFL AWVCFLFYAG IALFTSGFLL TRLELTNHSS CQEPPGPGSL PWGSQGKPGA 60
CWMASRFSRV VLVLIDALRF DFAQPQHSHV PREPPVSLPF LGKLSSLQRI LEIQPHHARL 120
YRSQVDPPTT TMQRLKALTT GSLPTFIDAG SNFASHAIVE DNLIKQLTSA GRRVVFMGDD 180
TWKDLFPGAF SKAFFFPSFN VRDLDTVDNG ILEHLYPTMD SGEWDVLIAH FLGVDHCGHK 240
HGPHHPEMAK KLSQMDQVIQ GLVERLENDT LLVVAGDHGM TTNGDHGGDS ELEVSAALFL 300
YSPTAVFPST PPEEPEVIPQ VSLVPTLALL LGLPIPFGNI GEVMAELFSG GEDSQPHSSA 360
LAQASALHLN AQQVSRFLHT YSAATQDLQA KELHQLQNLF SKASADYQWL LQSPKGAEAT 420
LPTVIAELQQ FLRGARAMCI ESWARFSLVR MAGGTALLAA SCFICLLASQ WAISPGFPFC 480
PLLLTPVAWG LVGAIAYAGL LGTIELKLDL VLLGAVAAVS SFLPFLWKAW AGWGSKRPLA 540
TLFPIPGPVL LLLLFRLAVF FSDSFVVAEA RATPFLLGSF ILLLVVQLHW EGQLLPPKLL 600
TMPRLGTSAT TNPPRHNGAY ALRLGIGLLL CTRLAGLFHR CPEETPVCHS SPWLSPLASM 660
VGGRAKNLWY GACVAALVAL LAAVRLWLRR YGNLKSPEPP MLFVRWGLPL MALGTAAYWA 720
LASGADEAPP RLRVLVSGAS MVLPRAVAGL AASGLALLLW KPVTVLVKAG AGAPRTRTVL 780
TPFSGPPTSQ ADLDYVVPQI YRHMQEEFRG RLERTKSQGP LTVAAYQLGS VYSAAMVTAL 840
TLLAFPLLLL HAERISLVFL LLFLQSFLLL HLLAAGIPVT TPGPFTVPWQ AVSAWALMAT 900
QTFYSTGHQP VFPAIHWHAA FVGFPEGHGS CTWLPALLVG ANTFASHLLF AVGCPLLLLW 960
PFLCESQGLR KRQQPPGNEA DARVRPEEEE EPLMEMRLRD APQHFYAALL QLGLKYLFIL 1020
GIQILACALA ASILRRHLMV WKVFAPKFIF EAVGFIVSSV GLLLGIALVM RVDGAVSSWF 1080
RQLFLAQQR 1089 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
 GO:0006501; P:C-terminal protein lipidation; TAS:Reactome.
 GO:0016254; P:preassembly of GPI anchor in ER membrane; TAS:Reactome. 
Interpro
 IPR017849; Alkaline_Pase-like_a/b/a.
 IPR017850; Alkaline_phosphatase_core.
 IPR002591; Phosphodiest/P_Trfase. 
Pfam
 PF01663; Phosphodiest 
SMART
  
PROSITE
  
PRINTS