UniProt Accession

 PACN1_RAT; Q9Z0W5 

Genbank Protein ID

 AF104402 

Genbank Nucleotide ID

 AAD16887.1 

Protein Name

 Protein kinase C and casein kinase substrate in neurons protein 1 

Protein Synonyms/Alias

 Dynamin proline-rich domain-interacting protein; Dynamin PRD-interacting protein; Synaptic, dynamin-associated protein I; Syndapin 1; Syndapin-I; SdpI 

Gene Name

 Pacsin1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
99	SELHQEVKNSLLNED	ubiquitination	[1]
187	VTPEQQKKLVDKVDK	acetylation	[2]
191	QQKKLVDKVDKCRQD	acetylation	[2]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Binds to membranes via its F-BAR domain and mediates membrane tubulation. Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accomodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission. 

Sequence Annotation

 DOMAIN 10 73 FCH.
 DOMAIN 382 441 SH3.
 REGION 1 304 F-BAR domain.
 MOD_RES 76 76 Phosphoserine.
 MOD_RES 181 181 Phosphothreonine.
 MOD_RES 391 391 Phosphotyrosine (By similarity).  

Keyword

 Cell junction; Cell membrane; Cell projection; Coiled coil; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Direct protein sequencing; Endocytosis; Lipid-binding; Membrane; Phosphoprotein; Reference proteome; SH3 domain; Synapse; Synaptosome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 441 AA 

Protein Sequence

Gene Ontology

 GO:0043679; C:axon terminus; IDA:UniProtKB.
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL.
 GO:0032587; C:ruffle membrane; ISS:UniProtKB.
 GO:0045202; C:synapse; IDA:BHF-UCL.
 GO:0005543; F:phospholipid binding; IDA:UniProtKB.
 GO:0007010; P:cytoskeleton organization; IDA:RGD.
 GO:0090002; P:establishment of protein localization to plasma membrane; IDA:BHF-UCL.
 GO:0097320; P:membrane tubulation; IMP:UniProtKB.
 GO:0048812; P:neuron projection morphogenesis; IMP:UniProtKB.
 GO:1900006; P:positive regulation of dendrite development; IDA:BHF-UCL.
 GO:0048488; P:synaptic vesicle endocytosis; ISS:UniProtKB. 

Interpro

 IPR001060; FCH_dom.
 IPR001452; SH3_domain. 

Pfam

 PF00611; FCH
 PF00018; SH3_1 

SMART

 SM00055; FCH
 SM00326; SH3 

PROSITE

 PS50133; FCH
 PS50002; SH3 

PRINTS

 PR00452; SH3DOMAIN.