| Tag | Content |
|---|
| CPLM ID | CPLM-021101 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Myosin phosphatase Rho-interacting protein |
Protein Synonyms/Alias | Rho-interacting protein 3; RIP3; p116Rip |
Gene Name | Mprip |
Gene Synonyms/Alias | Mrip; Rhoip3 |
Created Date | July 27, 2013 |
Organism | Rattus norvegicus (Rat) |
NCBI Taxa ID | 10116 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 400 | FKKGWLTKQYEDGQW | ubiquitination | [1] |
|
Reference | [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis. Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J. J Proteome Res. 2012 Sep 7;11(9):4722-32. [ PMID: 22871113] |
Functional Description | Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F- actin-binding region leads to disassembly of stress fibers in neuronal cells (By similarity). |
Sequence Annotation | DOMAIN 43 150 PH 1.
DOMAIN 391 487 PH 2.
REGION 1 387 Interaction with F-actin (By similarity).
REGION 550 828 Interaction with RHOA (By similarity).
REGION 828 883 Interaction with PPP1R12A (By
MOD_RES 198 198 Phosphoserine (By similarity).
MOD_RES 226 226 Phosphoserine (By similarity).
MOD_RES 230 230 Phosphoserine (By similarity).
MOD_RES 232 232 Phosphoserine (By similarity).
MOD_RES 275 275 Phosphoserine (By similarity).
MOD_RES 294 294 Phosphoserine (By similarity).
MOD_RES 297 297 Phosphoserine (By similarity).
MOD_RES 300 300 Phosphothreonine (By similarity).
MOD_RES 325 325 Phosphothreonine (By similarity).
MOD_RES 363 363 Phosphoserine (By similarity).
MOD_RES 369 369 Phosphoserine (By similarity).
MOD_RES 490 490 Phosphoserine (By similarity).
MOD_RES 622 622 Phosphoserine (By similarity).
MOD_RES 981 981 Phosphoserine (By similarity).
MOD_RES 997 997 Phosphoserine (By similarity).
MOD_RES 1018 1018 Phosphoserine (By similarity).
MOD_RES 1020 1020 Phosphoserine (By similarity). |
Keyword | Actin-binding; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Phosphoprotein; Reference proteome; Repeat. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1029 AA |
Protein Sequence | MSAAKENPCR KFQANIFNKS KCQNCFKPRE SHLLNDEDLT QAKPIYGGWL LLAPDGTDFD 60
NPVHRSRKWQ RRFFILYEHG LLRYALDEMP TTLPQGTINM NQCTDVVDGE ARTGQKFSLC 120
ILTPDKEHFI RAETKEIISG WLEMLMVYPR TNKQNQKKKR KVEPPTPQEP GPAKMAVTSS 180
SGGSSGSSSS SSSSSIPSAE KVPTTKSTLW QEEMRAKDQP DGTSLSPVQS PSQSQPPAAC 240
TPRETGLDSK EDENILSGDR VDGGRKVRVE SGYFSLEKAK QDLRAEEQLP PLLSPPSPST 300
PHSRRSQVIE KFEALDIEKA EHMETNMLIL TTPSSDTRQG RSERRAIPRK RDFASETPTA 360
PLSDACPLSP HRRAKSLDRR STESSMTPDL LNFKKGWLTK QYEDGQWKKH WFVLADQSLR 420
YYRDSVAEEA ADLDGEINLS TCYDVTEYPV QRNYGFQIHT KEGEFTLSAM TSGIRRNWIQ 480
TIMKHVLPTS APDVTSSLPE GKNKSTSFDT CLRPSEKQEA EPGEPDPEQK KSRARERRRE 540
GRSKTFDWAE FRPIQQALAQ ERASTVGSSD SGDPGCLEAE PGELERERAR RREERRKRFG 600
MLDTNDGPGM EDTALRMDID RSPGLLGTPD LKTQNVHVEI EQRWHQVETT PLREEKQVPI 660
APLHLSLEDR SERLSTHELT SLLEKELEQS QKEASDLLEQ NRLLQDQLRV ALGREQSARE 720
GYVLQATCER GFAAMEETHQ KKIEDLQRQH QRELEKLREE KDRLLAEETA ATISAIEAMK 780
NAHREEMERE LEKSQRSQIS SINSDIEALR RQYLEELQSV QRELEVLSEQ YSQKCLENAH 840
LAQALEAERQ ALRQCQRENQ ELNAHNQELN NRLAAEITRL RTLLTREGGG ESTGLPLTQG 900
KDAYELEVLL RVKESEIQYL KQEISSLKDE LQTALRDKKY ASDKYKDIYT ELSIAKAKAD 960
CDISRLKEQL KAATEALGEK SPEGTTVSGY DIMKSKSNPD FLKKDRSCVT RQLRNIRSKS 1020
VIEQVSWDN 1029 |
Gene Ontology | |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |