CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019561
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ATPase family AAA domain-containing protein 5 
Protein Synonyms/Alias
 Chromosome fragility-associated gene 1 protein 
Gene Name
 ATAD5 
Gene Synonyms/Alias
 C17orf41; FRAG1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
467NLQLHTDKGSFLKEKacetylation[1]
510KETTFFLKEKQYQNRubiquitination[2]
560SSLCNNNKLSRKTSIubiquitination[1, 2]
570RKTSIPVKDIKLTQSacetylation[1]
624VQDNSQLKASTQKAAubiquitination[2, 3, 4]
847SGLPDLLKRQIAKKAubiquitination[2]
922EFSTLNSKLKSGNSAubiquitination[2]
970YFPLLLKKQIEHQVLubiquitination[2]
1152CAQELGFKIFEVNASacetylation[5]
1770ANLDNAWKRISVIKSubiquitination[3]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786
Functional Description
 Involved in DNA damage response. Involved in a RAD9A- related damage checkpoint, a pathway that is important in determining whether DNA damage is compatible with cell survival or whether it requires cell elimination by apoptosis. Modulates the RAD9A interaction with BCL2 and thereby induces DNA damages- induced apoptosis. 
Sequence Annotation
 NP_BIND 1132 1139 ATP (Potential).
 MOTIF 1428 1432 LXCXE motif.
 MOD_RES 44 44 Phosphoserine.
 MOD_RES 219 219 Phosphoserine.
 MOD_RES 306 306 Phosphoserine.
 MOD_RES 311 311 Phosphoserine.
 MOD_RES 356 356 Phosphoserine.
 MOD_RES 369 369 Phosphoserine.
 MOD_RES 614 614 Phosphoserine.
 MOD_RES 621 621 Phosphoserine.
 MOD_RES 1116 1116 Phosphoserine.  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; DNA damage; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1844 AA 
Protein Sequence
MVGVLAMAAA AAPPPVKDCE IEPCKKRKKD DDTSTCKTIT KYLSPLGKTR DRVFAPPKPS 60
NILDYFRKTS PTNEKTQLGK ECKIKSPESV PVDSNKDCTT PLEMFSNVEF KKKRKRVNLS 120
HQLNNIKTEN EAPIEISSDD SKEDYSLNND FVESSTSVLR YKKQVEVLAE NIQDTKSQPN 180
TMTSLQNSKK VNPKQGTTKN DFKKLRKRKC RDVVDLSESL PLAEELNLLK KDGKDTKQME 240
NTTSHANSRD NVTEAAQLND SIITVSYEEF LKSHKENKVE EIPDSTMSIC VPSETVDEIV 300
KSGYISESEN SEISQQVRFK TVTVLAQVHP IPPKKTGKIP RIFLKQKQFE MENSLSDPEN 360
EQTVQKRKSN VVIQEEELEL AVLEAGSSEA VKPKCTLEER QQFMKAFRQP ASDALKNGVK 420
KSSDKQKDLN EKCLYEVGRD DNSKKIMENS GIQMVSKNGN LQLHTDKGSF LKEKNKKLKK 480
KNKKTLDTGA IPGKNREGNT QKKETTFFLK EKQYQNRMSL RQRKTEFFKS STLFNNESLV 540
YEDIANDDLL KVSSLCNNNK LSRKTSIPVK DIKLTQSKAE SEASLLNVST PKSTRRSGRI 600
SSTPTTETIR GIDSDDVQDN SQLKASTQKA ANLSEKHSLY TAELITVPFD SESPIRMKFT 660
RISTPKKSKK KSNKRSEKSE ATDGGFTSQI RKASNTSKNI SKAKQLIEKA KALHISRSKV 720
TEEIAIPLRR SSRHQTLPER KKLSETEDSV IIIDSSPTAL KHPEKNQKKL QCLNDVLGKK 780
LNTSTKNVPG KMKVAPLFLV RKAQKAADPV PSFDESSQDT SEKSQDCDVQ CKAKRDFLMS 840
GLPDLLKRQI AKKAAALDVY NAVSTSFQRV VHVQQKDDGC CLWHLKPPSC PLLTKFKELN 900
TKVIDLSKCG IALGEFSTLN SKLKSGNSAA VFMRTRKEFT EEVRNLLLEE IRWSNPEFSL 960
KKYFPLLLKK QIEHQVLSSE CHSKQELEAD VSHKETKRKL VEAENSKSKR KKPNEYSKNL 1020
EKTNRKSEEL SKRNNSSGIK LDSSKDSGTE DMLWTEKYQP QTASELIGNE LAIKKLHSWL 1080
KDWKRRAELE ERQNLKGKRD EKHEDFSGGI DFKGSSDDEE ESRLCNTVLI TGPTGVGKTA 1140
AVYACAQELG FKIFEVNASS QRSGRQILSQ LKEATQSHQV DKQGVNSQKP CFFNSYYIGK 1200
SPKKISSPKK VVTSPRKVPP PSPKSSGPKR ALPPKTLANY FKVSPKPKNN EEIGMLLENN 1260
KGIKNSFEQK QITQTKSTNA TNSNVKDVGA EEPSRKNATS LILFEEVDVI FDEDAGFLNA 1320
IKTFMATTKR PVILTTSDPT FSLMFDGCFE EIKFSTPSLL NVASYLQMIC LTENFRTDVK 1380
DFVTLLTANT CDIRKSILYL QFWIRSGGGV LEERPLTLYR GNSRNVQLVC SEHGLDNKIY 1440
PKNTKKKRVD LPKCDSGCAE TLFGLKNIFS PSEDLFSFLK HKITMKEEWH KFIQLLTEFQ 1500
MRNVDFLYSN LEFILPLPVD TIPETKNFCG PSVTVDASAA TKSMNCLARK HSEREQPLKK 1560
SQKKKQKKTL VILDDSDLFD TDLDFPDQSI SLSSVSSSSN AEESKTGDEE SKARDKGNNP 1620
ETKKSIPCPP KTTAGKKCSA LVSHCLNSLS EFMDNMSFLD ALLTDVREQN KYGRNDFSWT 1680
NGKVTSGLCD EFSLESNDGW TSQSSGELKA AAEALSFTKC SSAISKALET LNSCKKLGRD 1740
PTNDLTFYVS QKRNNVYFSQ SAANLDNAWK RISVIKSVFS SRSLLYVGNR QASIIEYLPT 1800
LRNICKTEKL KEQGKSKRRF LHYFEGIHLD IPKETVNTLA ADFP 1844 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0017111; F:nucleoside-triphosphatase activity; IEA:InterPro.
 GO:0006974; P:response to DNA damage stimulus; IEA:UniProtKB-KW. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00004; AAA 
SMART
 SM00382; AAA 
PROSITE
 PS00674; AAA 
PRINTS